SMPD4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NXE4

Core Product Specifications and Parameters

Biological Background: SMPD4 Function and Localization

• SMPD4 (Sphingomyelin phosphodiesterase 4), also known as nSMase-3, SKNY, NET13, is encoded by the SMPD4 gene and produces isoforms via alternative splicing.
• Catalyzes the hydrolysis of sphingomyelin to ceramide and phosphorylcholine, regulating membrane sphingolipid homeostasis (PubMed:16517606, PubMed:25180167). Related references: PMID:16517606, PMID:25180167
• Plays a role in endoplasmic reticulum organization and function, influencing membrane integrity (PubMed:31495489). Related references: PMID:31495489
• May sensitize cells to DNA damage-induced apoptosis (PubMed:18505924). Related references: PMID:18505924
• In skeletal muscle, mediates TNF-stimulated oxidant production (By similarity).
• Localizes to the endoplasmic reticulum membrane, Golgi apparatus membrane, nuclear envelope, cell membrane, and sarcolemma.
• Widely expressed, with highest levels in heart and skeletal muscle; detected in skeletal muscle at the protein level.
• Associated with intellectual disability (disease variant).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 600-800 of human SMPD4 (NP_060421.2). For assay optimization, consider performing epitope mapping or using this region for specificity controls.
• SMPD4 is a multi-pass transmembrane protein with a predicted molecular weight of ~98 kDa. Ensure appropriate membrane protein extraction protocols and include positive controls (e.g., heart or skeletal muscle lysates).
• For Western blot, consider using a reducing agent and heating to ensure full denaturation of the transmembrane domains.
• The antibody is validated for WB, IF/ICC, and ELISA with human and mouse samples. Perform cross-reactivity validation in your specific experimental model.
• For IF/ICC, permeabilization may be required to detect ER/Golgi/nuclear envelope localization; suitable fixation methods should be tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this SMPD4 antibody, and bulk ancillary reagents. Our sourcing services support diagnostic manufacturers and researchers studying sphingolipid metabolism and membrane biology. From concept to clinic, we offer one-stop access to rare target raw materials and technical support.