SMN2 Rabbit pAb - Q16637

Core Product Specifications and Parameters

Biological Background: Survival Motor Neuron Protein Function and Localization

• Survival motor neuron protein (SMN), also known as Gemin-1, is encoded by the SMN1 and SMN2 genes and is a core component of the gemin complex.
• The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Related references: PMID:18984161, PMID:9845364
• SMN acts as a structural backbone; together with GEMIN2, it gathers Sm proteins and orchestrates the stepwise assembly of the Sm core on snRNA, ensuring proper snRNP biogenesis. Related references: PMID:17178713, PMID:21816274, PMID:22101937
• It ensures the correct splicing of U12 intron-containing genes that are critical for normal motor and proprioceptive neuron development. Related references: PMID:23063131
• SMN is also required for resolving RNA–DNA hybrids (R-loops) created by RNA polymerase II in transcription terminal regions, facilitating proper transcription termination. Related references: PMID:26700805
• Subcellular localization includes nuclear gems and Cajal bodies, as well as cytoplasmic granules, neuronal projections, axons, and even the sarcomeric Z line in muscle cells.
• It is expressed in a wide variety of tissues, with high levels in brain, kidney, liver, and spinal cord, moderate levels in skeletal and cardiac muscle, and low levels in fibroblasts and lymphocytes.
• Key post-translational modifications include phosphorylation and acetylation; the protein is also ubiquitinated and undergoes alternative splicing, with links to neurodegeneration and motor neuron diseases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–197) shared by SMN1 and SMN2; the antibody likely recognizes both isoforms. Consider validating specificity using knockout or knockdown systems if isoform distinction is required.
• For Western blot, a band at approximately 32 kDa is expected; post-translational modifications may cause slight shifts in mobility. Include appropriate positive and negative controls.
• In immunofluorescence/ICC, expect nuclear gem/Cajal body puncta along with diffuse cytoplasmic staining. Optimize permeabilization conditions for the cell type under study.
• For immunoprecipitation, the antibody can precipitate native SMN complexes; confirm co-precipitation of known interactors such as GEMIN2.
• ELISA applications should be titrated under standard conditions; consider pairing with a capture antibody for sandwich formats.

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