SMC2 Rabbit Polyclonal Antibody for WB and ELISA - O95347

Core Product Specifications and Parameters

Biological Background: SMC2 Function and Localization

• SMC2, also known as hCAP-E or XCAP-E homolog, is a structural maintenance of chromosomes protein encoded by the SMC2 gene in humans.
• It serves as a central component of the condensin complex, which is essential for converting interphase chromatin into condensed mitotic chromosomes.
• The condensin complex likely introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms with type II topoisomerases.
• SMC2 belongs to the SMC protein family, which plays critical roles in chromosome dynamics, cohesion, and condensation throughout the cell cycle.
• Subcellular localization is predominantly nuclear and chromosome-associated, although cytoplasmic presence has also been reported.
• Key post-translational modifications include acetylation; the protein contains ATP-binding and nucleotide-binding domains characteristic of the SMC family.
• Functional keywords emphasize its involvement in DNA condensation, mitosis, cell division, and its coiled-coil structural motif.

Experimental Guidance and Technical Tips

• For western blot (WB), SMC2 runs at approximately 136 kDa. Consider using a low-percentage gel and extended transfer times for efficient detection of this large protein.
• Nuclear extract preparations may enhance the signal due to SMC2's primary localization; validate detection in your sample system of interest.
• In ELISA, inclusion of the recombinant immunogen (amino acids 700-900) as a positive control may assist in assay optimization.
• Cross-reactivity with mouse SMC2 is reported, making it suitable for studies using mouse models; however, sequence alignment of the immunogen region is advised to confirm conservation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a wide range of antibodies, antigens, and ancillary reagents to support diagnostic development and life science research. For chromosome biology and cell cycle projects, we can supply validated antibody pairs, bulk polyclonal antibodies like this SMC2 pAb, and assist with sourcing rare or specialized targets. Whether you need a single reagent or an end-to-end solution, CamelBio bridges your requirements from concept to clinic.