Smad2 Rabbit pAb - Q15796

Core Product Specifications and Parameters

Biological Background: Smad2 Function and Localization

• SMAD2 is the official gene symbol for SMAD family member 2, also known as MADH2, MADR2, JV18-1, and hMAD-2. The protein is a receptor-regulated SMAD (R-SMAD) that functions as an intracellular signal transducer for TGF-beta and activin signaling pathways.
• Upon ligand binding, type 1 receptor kinases phosphorylate SMAD2, leading to its association with SMAD4 and translocation to the nucleus where the complex binds to TRE elements in target gene promoters, activating transcription.
• SMAD2 positively regulates PDPK1 kinase activity by promoting its dissociation from the 14-3-3 protein YWHAQ, which otherwise acts as an inhibitor.
• SMAD2 may function as a tumor suppressor in colorectal carcinoma. Related references: PMID:8752209
• The protein localizes to both the cytoplasm and nucleus, shuttling between these compartments in response to signaling cues.
• High expression is found in skeletal muscle, endothelial cells, heart, and placenta.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitin-like conjugation, which regulate its activity and stability.
• Alternative splicing generates isoforms with potentially distinct regulatory properties.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 52 kDa. Consider using positive control lysates from human or mouse tissues with high Smad2 expression, such as skeletal muscle or placenta.
• For immunohistochemistry (paraffin-embedded), optimize antigen retrieval and antibody dilution. The protein localizes to both cytoplasm and nucleus, so staining may be observed in these compartments.
• For ELISA, pair with a matched detection antibody for optimal sensitivity. Validate cross-reactivity with mouse samples if required.
• Since Smad2 is activated by phosphorylation, consider using phospho-specific detection methods to study its activation state in TGF-beta signaling pathways.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for sourcing high-quality IVD raw materials, including this Smad2 rabbit polyclonal antibody. We supply validated antibodies, recombinant proteins, and ancillary reagents to accelerate your TGF-beta signaling research and diagnostic assay development. From target selection to bulk reagent supply, CamelBio streamlines your procurement process.