Smac/Diablo Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9NR28

Core Product Specifications and Parameters

Biological Background: Smac/Diablo Function and Localization

• The DIABLO (SMAC) gene encodes a mitochondrial protein that promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. It opposes inhibitor of apoptosis proteins (IAPs), including BIRC6/BRUCE, caspase-9, and XIAP/BIRC4. Related references: PMID:15200957 PMID:36758104 PMID:36758105
• Smac/Diablo attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting its ubiquitination and proteasomal degradation, and it disrupts XIAP/caspase-9 interaction to facilitate caspase-3 activation.
• Alternative splicing produces multiple isoforms; isoform 3 is ubiquitously expressed and may be defective in XIAP down-regulation.
• Subcellularly, Smac/Diablo localizes to the mitochondrion and is released into the cytosol upon apoptotic stimuli.
• The protein is ubiquitously expressed with highest levels in testis; also high in heart, liver, kidney, spleen, prostate, and ovary; low in brain, lung, thymus, and peripheral blood leukocytes.
• Keywords: 3D-structure, Alternative splicing, Apoptosis, Cytoplasm, Deafness, Direct protein sequencing, Disease variant, Mitochondrion, Non-syndromic deafness, Ubl conjugation.

Experimental Guidance and Technical Tips

• The recombinant immunogen (aa 56-239) corresponds to the mature processed region expected after mitochondrial cleavage; the antibody may detect both full-length (27 kDa) and processed forms (21/22 kDa).
• For Western blot, consider loading appropriate positive control lysates from human, mouse, or rat tissues, and optimize reducing conditions to resolve distinct isoforms.
• In IHC-P and IF/ICC, antigen retrieval (e.g., citrate buffer pH 6.0) and permeabilization should be optimized; cytoplasmic/mitochondrial staining patterns may require careful validation.
• ELISA applications require individual titration of capture and detection reagents; the antibody can be evaluated as part of a matched pair after functional testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated sourcing partner for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and custom development services. For Smac/Diablo research, we supply highly characterized monoclonal antibodies like this clone, along with recombinant proteins, antigen standards, and matched detection reagents to accelerate your apoptosis assay workflow. Reach out to explore comprehensive, one-stop solutions for your diagnostic and research needs.