SLCO1B1 Rabbit pAb - Q9Y6L6

Core Product Specifications and Parameters

Biological Background: SLCO1B1 Function and Localization

• SLCO1B1 mediates sodium-independent uptake of organic anions, functioning as a polyspecific transporter in the liver. Related references: PMID:10358072, PMID:15159445, PMID:17412826

• It transports endogenous substrates including bile acids (taurocholate), conjugated steroids (17-beta-glucuronosyl estradiol, dehydroepiandrosterone sulfate, estrone 3-sulfate), eicosanoids (prostaglandin E2, thromboxane B2, leukotriene C4, leukotriene E4), and thyroid hormones (T4, T3). Related references: PMID:10358072, PMID:10601278, PMID:10873595

• The transporter plays a key role in hepatic drug clearance, taking up statins such as pravastatin and pitavastatin, the chemotherapeutic methotrexate, and antihypertensives like enalapril and valsartan. Related references: PMID:10601278, PMID:15159445, PMID:15970799, PMID:23243220, PMID:16624871, PMID:16627748

• SLCO1B1 contributes to the liver-blood detoxification shuttle by transporting bilirubin glucuronides from plasma into hepatocytes. Related references: PMID:22232210

• Transport activity is pH-sensitive; an acidic microenvironment stimulates substrate uptake, and bicarbonate acts as the likely counteranion. Related references: PMID:19129463

• The protein is localized to the basolateral (sinusoidal) membrane of hepatocytes, consistent with its role in hepatic uptake.

• It is highly liver-specific, with expression restricted to centrilobular hepatocytes and weak expression in testicular Sertoli cells.

• SLCO1B1 undergoes post-translational modifications including N-glycosylation and phosphorylation, and contains disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 597-691), which may provide specificity for SLCO1B1; however, cross-reactivity with homologous transporters should be assessed in the relevant sample system.
• For Western blot, a band near 76 kDa is expected in liver lysates; due to potential glycosylation, the observed molecular weight might appear higher.
• In ELISA applications, pairing with an SLCO1B1 capture antibody or using recombinant protein as a standard can improve assay performance.
• Since SLCO1B1 expression is liver-specific, ensure positive control samples are from liver tissue or hepatocyte-derived cell lines.

CamelBio: Your One-Stop Sourcing Bridge

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