SLC7A1 Rabbit pAb for WB, IHC-P, ELISA - P30825

Core Product Specifications and Parameters

Biological Background: SLC7A1 Function and Localization

• SLC7A1 (also known as CAT-1, ERR, ATRC1, REC1L) encodes the high affinity cationic amino acid transporter 1, a ~68 kDa transmembrane glycoprotein.
• It functions as a high-affinity, low-capacity permease that mediates the cellular uptake of cationic amino acids such as arginine, lysine, and ornithine, primarily in non-hepatic tissues.
• The protein belongs to the solute carrier family 7 and also serves as a receptor for ecotropic retroviruses, enabling viral entry.
• Subcellular localization is at the cell membrane, where it acts as a multi-pass transmembrane protein.
• Expression is ubiquitous, detected across various tissues, reflecting its fundamental role in amino acid transport.
• Post-translational modifications include N-glycosylation, which may affect its trafficking and function.

Experimental Guidance and Technical Tips

• The immunogen was designed against a synthetic peptide spanning amino acids 530–629 within the C-terminal region of human SLC7A1, which may reduce cross-reactivity with closely related transporter family members.
• For Western blotting (WB), expect a major band at approximately 68 kDa; due to possible glycosylation or dimerization, additional bands may appear, and validation in the relevant sample system is recommended.
• For immunohistochemistry (IHC-P), antigen retrieval conditions may need optimization, and appropriate controls should be included to confirm staining specificity.
• For ELISA, the antibody can be used as a capture or detection reagent; pairing with a matched antibody is advised for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio offers diagnostic manufacturers and laboratories a streamlined procurement channel for validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For SLC7A1 research and assay development, we can support your projects with custom antibody sourcing and technical consultation, ensuring a reliable supply of raw materials from concept to clinical application.