SLC25A6 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P12236

Core Product Specifications and Parameters

Biological Background: SLC25A6/ADP-ATP Translocase 3 Function and Localization

• SLC25A6 encodes the ADP/ATP translocase 3 (ANT3), a member of the solute carrier family 25, with alternative names including adenine nucleotide translocator 3, ANT2, AAC3, and ANT3Y.
• It functions as an ADP:ATP antiporter, mediating the import of ADP into the mitochondrial matrix and export of ATP, operating via an alternating access mechanism with cytoplasmic-open and matrix-open states.
• The protein also contributes to mitochondrial uncoupling and plays a key role in mitochondrial permeability transition pore (mPTP) opening, processes linked to apoptosis and energy homeostasis (PubMed:15033708). Related references: PMID:15033708
• Additionally, SLC25A6 exhibits proton transporter activity, which requires free fatty acids as cofactors and is inhibited by its ADP:ATP antiporter function, thereby regulating thermogenesis.
• Localized to the mitochondrial inner membrane, it acts as a master regulator balancing ATP production and thermogenesis.
• Expressed in erythrocytes at the protein level, the protein undergoes post-translational modifications including acetylation and methylation, and is implicated in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 100-200 of human SLC25A6; the polyclonal antibody is expected to recognize both native and denatured protein, making it suitable for WB and IF/ICC applications.
• For WB, a band at approximately 33 kDa is anticipated; validate the antibody in your specific lysates, and consider using mitochondrial extracts as positive controls.
• In IF/ICC, mitochondrial localization should be observed; co-staining with a mitochondrial marker is recommended to confirm specificity.
• ELISA optimization can utilize the peptide immunogen as a positive control.
• Cross-reactivity with mouse and rat suggests a conserved epitope; validation in these species is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research teams a streamlined, one-stop channel for IVD raw-material sourcing, including validated polyclonal antibodies like this anti-SLC25A6 antibody. With expertise in mitochondrial biology and apoptosis targets, we supply optimized antibody pairs, bulk ancillary reagents, and support the procurement of rare-target raw materials. Reach out to discuss how we can meet your specific assay development and supply needs.