SLC25A13 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UJS0

Core Product Specifications and Parameters

Biological Background: SLC25A13 Function and Localization

• SLC25A13 (citrin, ARALAR2) is a mitochondrial carrier protein of the solute carrier family 25, functioning as an electrogenic aspartate/glutamate antiporter essential for the malate-aspartate shuttle and NADH redox balance across the inner mitochondrial membrane. Related references: PMID:11566871, PMID:38937634, PMID:38945283
• Substrate exchange occurs via a sequential mechanism in which one substrate binds and dissociates before the counter-substrate binds for transport in the opposite direction. Related references: PMID:38937634
• In addition to aspartate/glutamate antiport, SLC25A13 mediates mitochondrial uptake of L-cysteinesulfinate in exchange for glutamate and a proton, or can exchange L-cysteinesulfinate with aspartate without proton translocation. Related references: PMID:11566871
• SLC25A13 is localized to the mitochondrial inner membrane and is highly expressed in liver, with lower expression in kidney, pancreas, placenta, heart, and brain.
• The protein undergoes post-translational modifications including acetylation, phosphorylation, and methylation, and contains calcium-binding domains.
• Disease variants in SLC25A13 are associated with intrahepatic cholestasis and other metabolic disorders.

Experimental Guidance and Technical Tips

• The immunogen covers the N‑terminal 1–300 amino acids of human SLC25A13 (isoform NP_001153682.1). Verify isoform expression in the relevant sample system, as this region may differ in alternative transcripts.
• For Western blot, a band near 74 kDa is expected. Use mitochondrial‑enriched or whole‑cell lysates from liver (high expression) and validate with appropriate controls.
• IHC‑P applications should include liver sections as a positive control; optimized antigen retrieval and detection conditions may be necessary. Consider antibody titration to reduce background.
• Polyclonal antibodies offer sensitive detection in ELISA. Cross‑reactivity with the mouse ortholog is reported; if using mouse models, confirm specificity with appropriate knockout or knockdown controls.
• As with any experimental procedure, consult relevant literature and validate the antibody’s performance in the specific application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic kit developers and research laboratories with a comprehensive range of IVD raw materials, including validated antibodies, antigen pairs, and ancillary reagents. For research into mitochondrial transporters such as SLC25A13 (citrin), we provide rabbit polyclonal antibodies and can assist with sourcing matched detection reagents, recombinant proteins, and other critical materials to accelerate assay development and validation.