SKAP2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75563

Core Product Specifications and Parameters

Biological Background: SKAP2 Function and Localization

• SKAP2 (Src kinase-associated phosphoprotein 2), also designated SKAP55HOM, SCAP2, or SKAP55R, is an adaptor protein with N-terminal pleckstrin homology (PH) and C-terminal SH3 domains.
• It functions in coupling B-cell receptor (BCR) signaling to integrin activation, suggesting a role in B-cell adhesion and migration.
• SKAP2 may also contribute to macrophage adhesion processes, implicating it in innate immune cell trafficking.
• It is thought to participate in src family kinase signaling, linking upstream tyrosine phosphorylation events to downstream cytoskeletal rearrangements.
• Subcellularly, SKAP2 localizes predominantly to the cytoplasm.
• Ubiquitously expressed across tissues, with confirmed presence at the protein level in platelets.
• Annotated as a phosphoprotein and reference proteome component, with resolved 3D structural data available.

Experimental Guidance and Technical Tips

• The immunogen (aa 1–120 of human SKAP2) lies at the N-terminus, likely to recognize full-length protein in denaturing and native conditions; consider verifying reactivity against endogenous protein in relevant cell lines.
• For WB, use whole-cell lysates from human, mouse, or rat B-cell or macrophage lines as positive controls; expected band ~41 kDa.
• IF/ICC experiments may require permeabilization due to cytoplasmic localization; validate antibody specificity with appropriate knockout or siRNA controls in the target species.
• ELISA coating conditions and detection range should be optimized using recombinant SKAP2 protein or cell lysates to account for polyclonal lot variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibodies, reagents, and technical consultation. This SKAP2 antibody, covering immunology and cell adhesion targets, aligns with our capability to supply optimized polyclonal antibodies and ancillary materials for immunoassay development. Reach out to explore bulk orders or custom sourcing for rare signaling targets.