SIRT6 Rabbit Polyclonal Antibody for WB and ELISA - Q8N6T7

Core Product Specifications and Parameters

Biological Background: SIRT6 Function and Localization

• SIRT6 is a NAD-dependent protein deacetylase, deacylase, and mono-ADP-ribosyltransferase that plays essential roles in DNA damage repair, telomere maintenance, metabolic homeostasis, inflammation, tumorigenesis, and aging. Related references: PMID:18337721, PMID:19135889, PMID:19625767
• SIRT6 deacetylates histone H3 at Lys9, Lys18, and Lys56, suppressing NF-kappa-B target gene expression, inhibiting transcription elongation, and maintaining pericentric heterochromatin silencing at centromeres to prevent genomic instability. Related references: PMID:19625767, PMID:21362626, PMID:23892288, PMID:27043296
• SIRT6 promotes DNA double-strand break repair by recognizing and binding DSB sites, recruiting chromatin remodelers such as SMARCA5, and deacetylating non-histone repair proteins like DDB2 and p53. Related references: PMID:23911928, PMID:31995034, PMID:32538779, PMID:29474172, PMID:32789493
• At telomeres, SIRT6 deacetylates histone H3 to regulate telomere position effect and telomere movement in response to DNA damage, contributing to telomere maintenance. Related references: PMID:18337721, PMID:19625767, PMID:21847107
• SIRT6 regulates cellular metabolism by inhibiting glycolysis through HIF1A co-repression and PKM2 deacetylation, suppressing the Warburg effect, and modulating gluconeogenesis via FOXO1 and GCN5 deacetylation. Related references: PMID:24012758, PMID:26787900, PMID:23217706, PMID:23142079, PMID:25009184
• SIRT6 localizes predominantly to the nucleus and is enriched at chromosomes and telomeres; it also has a presence at the endoplasmic reticulum, consistent with its role in protein secretion.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from mouse SIRT6; cross-reactivity with human SIRT6 is reported. Validate species-specific detection in relevant cell or tissue lysates.
• For Western blot, mouse SIRT6 migrates at approximately 36 kDa, and human SIRT6 at 39 kDa. Include appropriate positive controls (e.g., mouse or human cell lines expressing SIRT6) and consider using nuclear-enriched fractions for optimal detection.
• For ELISA, optimize coating antigen and primary antibody concentration using recombinant SIRT6 protein or the immunizing peptide as a standard.
• Because SIRT6 is a chromatin-associated nuclear protein, efficient extraction for WB may require sonication or nuclear isolation protocols.
• Performance in other applications (e.g., IHC, IF, ChIP) has not been tested; conduct pilot experiments if desired.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated access to validated IVD raw materials, including polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents, supporting diagnostic manufacturers and research laboratories from concept to clinic. This SIRT6 rabbit polyclonal antibody is suitable for studies in aging, cancer metabolism, DNA damage repair, and epigenetic regulation. For bulk orders, custom antibody development, or complementary reagents, please contact our sourcing team for tailored solutions.