SIRT2 Rabbit Polyclonal Antibody for WB, ELISA - Q8IXJ6

Core Product Specifications and Parameters

Biological Background: SIRT2 Function and Localization

• SIRT2 is an NAD-dependent protein deacetylase that removes acetyl groups from histones (e.g., H4K16, H3K56) and alpha-tubulin, modulating chromatin structure and microtubule dynamics. Related references: PMID:12620231, PMID:16648462, PMID:18249187
• It plays a critical role in cell cycle progression by acting in the antephase checkpoint and promoting mitotic exit through APC/C complex activation. Related references: PMID:12697818, PMID:16909107, PMID:17488717, PMID:22014574
• SIRT2 regulates transcription by deacetylating transcription factors such as FOXO1, HIF1A, RELA, p53, and EP300, influencing gene expression in metabolism, hypoxia, and inflammation. Related references: PMID:18249187, PMID:18995842, PMID:20543840, PMID:24681946, PMID:21081649, PMID:22771473
• It is involved in metabolic regulation, including glucose homeostasis via PCK1 stabilization, pentose phosphate pathway activation through G6PD, and lipid metabolism by inhibiting adipogenesis and promoting lipolysis. Related references: PMID:21726808, PMID:24769394, PMID:20543840
• SIRT2 functions as a tumor suppressor, with expression downregulated in gliomas and several other cancers.
• Subcellularly, SIRT2 shuttles between nucleus and cytoplasm, localizing to chromatin, cytoskeleton, centrosome, spindle, and midbody during cell division.
• Tissue‐specific expression: isoform 1 is found in heart, liver, and skeletal muscle, while isoform 2 is predominantly expressed in the cortex; expression is generally downregulated in cancer tissues.
• Post‐translational modification: phosphorylation at Ser‑368 by cyclin E‑CDK2 inactivates SIRT2, negatively regulating cell adhesion and migration. Related references: PMID:17488717

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to a region within amino acids 300–350 of human SIRT2; consider that the antibody may preferentially recognize epitopes in this C‑terminal region.
• For Western blot, validate detection in lysates from human, mouse, and rat cell lines or tissues, keeping in mind the predicted molecular weight of ~43 kDa.
• In ELISA applications, the peptide immunogen can serve as a coating antigen for antibody titration; however, empirical optimization of coating conditions and concentrations is recommended.
• Because SIRT2 shuttles between subcellular compartments, subcellular fractionation may help enrich the target and improve detection sensitivity in certain experimental setups.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, supporting diagnostic manufacturers and researchers from early development to clinical application. This anti‑SIRT2 polyclonal antibody is part of our expanding catalog of high‑quality antibodies for epigenetic and cancer research, and we also provide validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary reagents, and customized sourcing for rare targets. Let CamelBio be your reliable partner in advancing precision diagnostics.