Siglec1 Rabbit pAb - Q62230

Core Product Specifications and Parameters

Biological Background: Siglec1 Function and Localization

• Sialoadhesin (Siglec-1, CD169) is a macrophage-restricted adhesion molecule that binds sialic acids on lymphocytes and pathogens, playing a key role in innate immune surveillance.
• It mediates sialic acid-dependent phagocytosis of bacteria to limit dissemination and supports antigen presentation for adaptive immunity. Related references: PMID:24788876
• Siglec-1 also recognizes sialylated enveloped viruses, leading to the formation of intracellular virion-containing compartments and enhancing viral transmission from macrophages to T cells. Related references: PMID:26370074
• Functions as an endocytic receptor via clathrin-dependent endocytosis, preferentially binding α2,3-linked sialic acids, and interacts with SPN/CD43 on T cells.
• It inhibits antiviral innate immunity by promoting TBK1 degradation through TYROBP and TRIM27-mediated ubiquitination.
• The protein is localized to the cell membrane and can be secreted; it is highly expressed in spleen and lymph node macrophages, particularly in subcapsular and interfollicular regions.
• Post-translational modifications include N-glycosylation and disulfide bonds, and the gene undergoes alternative splicing to produce multiple isoforms.
• Additional roles may include participation in hemopoiesis.

Experimental Guidance and Technical Tips

• For WB, expect a band around 183kDa; consider using a low-percentage gel and extended transfer times for large proteins.
• Validate antibody specificity in the relevant sample system (e.g., mouse macrophage lysates) and include appropriate positive and negative controls.
• For ELISA applications, the recombinant immunogen (amino acids 20–138) can serve as a reference standard.
• Due to documented cross-reactivity with rat, this antibody may be suitable for rat macrophage studies; confirm with pilot experiments in the target model.
• Pair this polyclonal antibody with a matched detection reagent to enable sandwich assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents tailored to macrophage and infectious disease research. This anti-Siglec1 product is part of our extensive immunoglobulin portfolio, supported by technical consulting and custom rare-target development to help you advance from concept to clinic.