Siglec-15 Rabbit pAb - Q6ZMC9

Core Product Specifications and Parameters

Biological Background: Siglec-15 Function and Localization

• Siglec-15, also known as CD33 antigen-like 3 (CD33L3) or HsT1361, is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family.
• It functions as a receptor that binds sialylated glycoproteins, likely contributing to immune cell signaling and adhesion.
• The protein is a single-pass type I membrane protein, featuring an extracellular N-terminal V-set immunoglobulin domain and a C2-set domain, a transmembrane helix, and a cytoplasmic tail.
• Siglec-15 is localized on the cell membrane.
• It is predominantly expressed in macrophage and/or dendritic cells of spleen and lymph nodes, implying a role in immune regulation.
• The SIGLEC15 gene undergoes alternative splicing, producing multiple isoforms.
• As indicated by UniProt keywords, Siglec-15 is a glycoprotein with a predicted molecular weight of ~35.6 kDa; the observed weight is 36 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering the extracellular region (amino acids 20-263), which includes the immunoglobulin domains; this design typically yields antibodies that recognize the native conformation.
• For Western blot (WB), a band at approximately 36 kDa is expected under reducing conditions; consider validating the antibody in lysates from spleen or lymph node tissues.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), test on paraffin-embedded sections or frozen sections of human spleen or lymph node, as Siglec-15 is expressed in macrophage/dendritic cells.
• For ELISA, the antibody can be used as capture or detection reagent; perform checkerboard titrations to determine optimal working concentrations.
• Cross-reactivity with rat has been reported; verify performance in your specific experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents. Our Siglec-15 Rabbit pAb supports research in immunology and immune-oncology biomarker development. From early-stage feasibility to commercial supply, CamelBio streamlines procurement, helping you reduce lead times and accelerate assay development.