SHMT2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P34897

Core Product Specifications and Parameters

Biological Background: SHMT2 Function and Localization

• SHMT2 (serine hydroxymethyltransferase, mitochondrial) is a pyridoxal phosphate-dependent transferase that catalyzes the reversible conversion of serine and tetrahydrofolate (THF) to glycine and 5,10-methylenetetrahydrofolate (5,10-CH2-THF), a critical one-carbon donor for purine and thymidylate biosynthesis. Related references: PMID:24075985, PMID:25619277, PMID:29364879
• SHMT2 localizes predominantly to the mitochondrial matrix and nucleoid, where it associates with mitochondrial DNA and contributes to mitochondrial translation by generating 5,10-CH2-THF for the modification of tRNA wobble uridine bases. Related references: PMID:18063578, PMID:29452640
• By supplying 5,10-CH2-THF, SHMT2 supports the de novo synthesis of thymidylate within mitochondria, preventing uracil accumulation in mitochondrial DNA and maintaining genomic integrity. Related references: PMID:21876188
• Beyond its enzymatic role, SHMT2 is a component of the BRISC deubiquitinase complex and is required for the removal of ubiquitin from IFNAR1, linking one-carbon metabolism to innate immune signaling. Related references: PMID:24075985
• SHMT2 is also detected in the cytoplasm and nucleus, and its expression is subject to alternative splicing; disease variants have been linked to cardiomyopathy and intellectual disability.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, which may regulate its activity and subcellular distribution.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 265-504) of human SHMT2, which is conserved in mouse and rat, supporting the reported cross-reactivity.
• For Western blot (WB), a band at approximately 56 kDa is expected in mitochondrial-enriched lysates.
• In immunohistochemistry (IHC) and immunofluorescence (IF/ICC), mitochondrial staining patterns should be observed; co-staining with mitochondrial markers is recommended for validation.
• For immunoprecipitation (IP), consider using native conditions as the antibody targets a folded domain.
• ELISA applications may require coating with recombinant SHMT2 protein.
• Always validate the antibody in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop sourcing partner for IVD raw materials, CamelBio provides high-quality antibodies like this SHMT2 polyclonal antibody to support one-carbon metabolism and mitochondrial disease research. Our extensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, ancillary reagents, and custom sourcing for rare targets, streamlining your assay development from concept to clinic.