SHMT1 Rabbit Polyclonal Antibody for WB, IHC, IF, ELISA - P34896

Core Product Specifications and Parameters

Biological Background: SHMT1 Function and Localization

• Serine hydroxymethyltransferase, cytosolic (SHMT1), also known as glycine hydroxymethyltransferase, is a pyridoxal phosphate (PLP)-dependent enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate (THF) to glycine and 5,10-methylene THF, serving as a critical component of the folate cycle and facilitating one-carbon biosynthetic reactions essential for methionine, purine, and pyrimidine synthesis. Related references: PMID:24698160, PMID:30035852, PMID:38996576

• SHMT1 is predominantly localized in the cytoplasm but also shuttles to the nucleus, where it plays a scaffold role essential for assembling the de novo thymidylate synthesis complex by anchoring DHFR and TYMS to the nuclear lamina and DNA replication sites. Related references: PMID:22235121

• Beyond its canonical role, SHMT1 catalyzes the cleavage of various 3-hydroxy amino acids, such as L-allo-threonine, L-threonine, and 3-phenylserine, forming glycine and the corresponding aldehyde; it also functions in the carnitine biosynthesis pathway as a hydroxytrimethyllysine aldolase (HTMLA). Related references: PMID:38615009

• SHMT1 possesses RNA-binding capability, forming complexes that selectively regulate SHMT2 mRNA translation and dynamically modulate cytosolic and mitochondrial serine and glycine concentrations, thereby influencing cellular metabolic status. Related references: PMID:38996576

• The SHMT1 gene undergoes alternative splicing, producing multiple isoforms that may exhibit distinct tissue-specific expression patterns and functional properties.

• Post-translational modifications, including acetylation and ubiquitination, are known to regulate SHMT1 activity and stability, as indicated by proteomics identification and UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 285–444 of human SHMT1, a region that may be conserved across species, supporting the observed cross-reactivity with mouse and rat. Validate reactivity in your sample system if using non-validated species.
• For Western blot, the predicted molecular weight is approximately 53 kDa, but confirm the band pattern using appropriate positive controls and consider possible isoforms arising from alternative splicing.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and fixation conditions, as SHMT1 localization (cytoplasmic and nuclear) may require different permeabilization strategies.
• Due to the polyclonal nature of this antibody, minor lot-to-lot variation may occur; include internal references for consistent quantification across experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For your SHMT1-related research and assay development, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our supply capabilities support projects in one-carbon metabolism, folate cycle, and cancer biology, ensuring you receive consistent, high-quality materials for your diagnostic and research workflows.