SHIP1 Rabbit pAb for WB, IF/ICC, IF-P, IHC-P, ELISA - Q92835

Core Product Specifications and Parameters

Biological Background: SHIP1 (INPP5D) Function and Localization

• SHIP1 (Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1), also known as INPP5D, SHIP, SHIP-1, hp51CN, SIP-145, and p150Ship, is a 133 kDa protein encoded by the INPP5D gene.
• It hydrolyzes PtdIns(3,4,5)P3 to PtdIns(3,4)P2, negatively regulating PI3K pathways. Related references: PMID:10764818 PMID:8723348 PMID:8769125
• It also hydrolyzes PtdIns(4,5)P3, Ins(1,4,5)P3, and Ins(1,3,4,5)P4. Related references: PMID:10764818 PMID:40969890 PMID:8769125
• SHIP1 negatively regulates B-cell antigen receptor signaling and mediates FCGR2B signaling to terminate activating immune receptor pathways.
• It controls myeloid cell proliferation, chemotaxis, mast cell degranulation, immune homeostasis, platelet integrin signaling, and JNK signaling in B-cells, as well as osteoclast precursor proliferation and macrophage function; it is required for endotoxin tolerance.
• SHIP1 is involved in cell-cell junction control, CD32a signaling in neutrophils, and modulation of EGF-induced phospholipase C activity. Related references: PMID:16682172
• Subcellular localization: cytoplasm, cell membrane, membrane rafts, cytoskeleton.
• Tissue specificity: predominantly expressed in immune and hematopoietic cells; high in immature CD34+ cells, low in mature CD33+ cells; majority in T-cells, minority in B-cells.
• Key features: phosphoprotein with SH2 domain and SH3-binding motif; undergoes alternative splicing; magnesium-dependent hydrolase involved in lipid metabolism and immunity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within the C-terminal region (aa 900–1000). This region may be present in some but not all splice variants; verify detection of your target isoform.
• Predicted molecular weight is ~133 kDa. For Western blot, consider using a 6–8% SDS-PAGE gel and optimize transfer for high molecular weight proteins.
• Reported cross-reactivity with human, mouse, and rat. However, validate the antibody in your species and tissue of interest.
• For immunofluorescence applications, expect localization to cytoplasm, cell membrane, and possibly cytoskeleton based on reported subcellular distribution.
• As a polyclonal antibody, lot-to-lot consistency may vary; include appropriate controls and consider validation with a known positive control lysate.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, including SHIP1 antibodies for immunology and hematology research. We offer validated antibody pairs, optimized polyclonal antibodies like this SHIP1 Rabbit pAb, bulk ancillary reagents, and rare target raw-material sourcing to support your diagnostic development needs.