SH2B1 Rabbit pAb for IHC-P, IF/ICC, ELISA - Q9NRF2

Core Product Specifications and Parameters

Biological Background: SH2B1 Function and Localization

• SH2B adapter protein 1 (SH2B1, also known as PSM) is an intracellular adapter protein that lacks intrinsic enzymatic activity but contains SH2 and PH domains, enabling recruitment to phosphotyrosine-containing receptors and adapters.
• It functions as a critical signaling mediator for multiple receptor tyrosine kinases (RTKs) and Janus kinase (JAK)-associated cytokine receptors, including insulin, insulin-like growth factor 1 (IGF1), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), platelet-derived growth factor (PDGF), and fibroblast growth factors (FGFs).
• In growth hormone (GH) and leptin signaling, SH2B1 binds to autophosphorylated JAK2 and enhances JAK2 kinase activity, promoting downstream pathways such as PI3K-Akt via IRS1/2 phosphorylation.
• Isoforms of SH2B1, generated by alternative splicing, can differentially modulate signaling; for instance, they may exhibit distinct roles in IGF1- and PDGF-induced mitogenesis.
• Subcellularly, SH2B1 localizes to the cytoplasm, cell membrane, and nucleus, consistent with its dynamic role in signal transduction.
• Post-translational modifications include phosphorylation on tyrosine and serine/threonine residues (e.g., by JAK2), which create docking sites for other signaling proteins, and methylation.
• SH2B1 is widely expressed across tissues, with the highest levels detected in skeletal muscle and ovary, reflecting its broad regulatory functions.

Experimental Guidance and Technical Tips

• For IHC-P, consider optimizing antigen retrieval and primary antibody dilution using positive control tissues such as skeletal muscle or ovary, where SH2B1 is highly expressed.
• For IF/ICC, fixation and permeabilization conditions should be empirically determined; the antibody may detect membrane, cytoplasmic, and/or nuclear pools depending on cell type and stimulation.
• Given the polyclonal nature of this antibody, it is advisable to perform a titration experiment and validate specificity using knockdown or knockout controls to account for potential batch-to-batch variability.
• ELISA may be used to detect endogenous SH2B1; however, appropriate standards and sample preparation protocols should be optimized for the specific assay format.
• This antibody is predicted to cross-react with mouse and rat SH2B1 based on sequence homology, but experimental validation in these species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This SH2B1 rabbit polyclonal antibody supports research in signal transduction, metabolic disorders, and neurobiology. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development and commercialization.