SF3B2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13435

Core Product Specifications and Parameters

Biological Background: SF3B2 Function and Localization

• SF3B2 (Splicing factor 3B subunit 2) is a component of the 17S U2 SnRNP complex of the major spliceosome, which catalyzes pre-mRNA splicing. Related references: PMID:12234937, PMID:32494006, PMID:34822310

• It participates in early spliceosome assembly and mediates recognition of the intron branch site, promoting selection of the branch-site adenosine for the first catalytic step. Related references: PMID:12234937, PMID:32494006, PMID:34822310

• As part of the SF3B subcomplex, SF3B2 is required for the formation of the spliceosomal A complex via stable U2 snRNP binding to the branchpoint sequence. Related references: PMID:12234937, PMID:27720643

• Together with SF3A, SF3B2 facilitates sequence-independent anchoring of U2 snRNP to pre-mRNA upstream of the branch site. Related references: PMID:12234937

• SF3B2 may also contribute to the assembly of the E complex, an early spliceosomal intermediate. Related references: PMID:10882114

• SF3B2 is additionally a component of the minor spliceosome, which processes U12-type introns. Related references: PMID:15146077, PMID:33509932

• Subcellularly, SF3B2 localizes to the nucleus and nuclear speckles, consistent with its role in pre-mRNA processing.

• Post-translational modifications include acetylation, methylation, and phosphorylation; the protein is associated with disease variants and host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 616–895, a region likely conserved across species, supporting the observed human, mouse, and rat cross-reactivity.
• For Western blotting, an expected band around 100 kDa should be observed; consider using a 6–8% SDS-PAGE gel for optimal separation and standard wet or semi-dry transfer conditions.
• IHC-P may require antigen retrieval (e.g., citrate buffer, pH 6.0); titrate antibody and validate in appropriate positive and negative control tissues.
• IF/ICC protocols should optimize fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) to detect nuclear speckle localization.
• ELISA performance is application‑dependent; pair with a suitable detection antibody and validate with recombinant or native SF3B2 protein.
• No single recommended dilution is provided; empirical optimization is essential for each application and sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to accelerate your assay development. This anti-SF3B2 polyclonal antibody supports research into spliceosome biology and may be integrated into your diagnostic workflows. Our team is ready to assist with your procurement needs and technical inquiries.