SETD6 Rabbit Monoclonal Antibody for WB and ELISA - Q8TBK2

Core Product Specifications and Parameters

Biological Background: SETD6 Function and Localization

• SETD6 (SET domain-containing protein 6) is an N-lysine methyltransferase that catalyzes the monomethylation of both histone and non-histone substrates.
• It downregulates NF-kappa-B transcription factor activity by monomethylating Lys-310 of the RELA (p65) subunit. Related references: PMID:21131967, PMID:21515635, PMID:30189201
• SETD6 monomethylates histone variant H2AZ at Lys-8 (H2AZK8me1), contributing to epigenetic regulation. Related references: PMID:23324626
• The enzyme is required for maintaining embryonic stem cell self-renewal through undefined methylation events.
• It also methylates the protein kinase PAK4, indicating involvement in cytoskeletal and signaling dynamics.
• SETD6 localizes predominantly to the nucleus, consistent with its role in transcriptional and chromatin regulation.
• The protein undergoes alternative splicing and features a characteristic SET methyltransferase domain; its molecular weight is predicted at ~53 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 310–473) of human SETD6, which may detect isoforms containing this sequence. Consider validating in the relevant species and sample types.
• For Western blot (WB), a band around 53 kDa is expected; optimization of loading controls and blocking conditions is recommended.
• For ELISA applications, pairing this antibody with a suitable detection antibody may require titration under specific assay conditions.
• Cross-reactivity with mouse and rat SETD6 should be verified in homologous tissues to confirm specificity.
• Since SETD6 is a nuclear protein, subcellular fractionation or nuclear extracts may enhance detection in certain experimental contexts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined sourcing pathway for IVD raw materials. This SETD6 antibody supports research into NF-kappa-B signaling and epigenetic modifications. Explore our portfolio for validated antibody pairs, bulk reagents, and rare target sourcing to accelerate your assay development.