SESN2 Rabbit pAb for WB, ELISA - P58004

Core Product Specifications and Parameters

Biological Background: SESN2 Function and Localization

• Functions as an intracellular leucine sensor that negatively regulates mTORC1 signaling through the GATOR complex. Related references: PMID:18692468 PMID:25263562 PMID:25457612
• Leucine binding to SESN2 disrupts its interaction with GATOR2, thereby activating TORC1 signaling. Related references: PMID:26449471 PMID:26586190 PMID:35114100
• May negatively regulate protein translation in response to endoplasmic reticulum stress, via mTORC1. Related references: PMID:24947615
• May positively regulate transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating SQSTM1-mediated autophagic degradation of KEAP1. Related references: PMID:23274085
• May prevent the accumulation of reactive oxygen species through N-terminal alkylhydroperoxide reductase activity; originally reported to contribute to oxidative stress resistance by reducing PRDX1, but this could not be confirmed. Related references: PMID:26612684 PMID:15105503 PMID:19113821
• Subcellular localization: Cytoplasm.
• Widely expressed across tissues.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bond/Ubl conjugation); also annotated as an oxidoreductase and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 211-480 of human SESN2; consider using full-length recombinant protein or lysates from cells expressing SESN2 for validation.
• For Western blotting, expect a band around 54 kDa; appropriate positive and negative controls are recommended to confirm specificity.
• ELISA may be used for quantitative detection; validate in the relevant sample system (e.g., human, mouse, or rat cell lysates) to ensure performance.
• As SESN2 is widely expressed, a variety of cell lines (e.g., HeLa, HEK293) may serve as suitable model systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop source for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. For research on SESN2—a key leucine sensor involved in mTORC1 signaling and oxidative stress—CamelBio provides seamless access to the critical reagents needed to advance your metabolic and stress biology studies.