SERPINC1 Rabbit pAb - P01008

Core Product Specifications and Parameters

Biological Background: SERPINC1 Function and Localization

• SERPINC1 encodes Antithrombin-III (Serpin C1), a secreted glycoprotein and member of the serpin superfamily.
• It functions as the principal plasma inhibitor of the coagulation cascade, blocking thrombin, matriptase-3/TMPRSS7, and factors IXa, Xa, and XIa. Related references: PMID:15140129 PMID:15853774
• Its inhibitory potency is markedly enhanced by heparin binding.
• Antithrombin-III is primarily produced in the liver and released into the blood, where it maintains hemostatic equilibrium.
• The mature protein is post-translationally modified by glycosylation and phosphorylation, resulting in an observed molecular weight of ca. 58 kDa.
• Key structural features include three disulfide bonds and a reactive center loop typical of serpins.
• Mutations in SERPINC1 are linked to thrombophilia and other bleeding disorders.
• The protein is conserved across human, mouse, and rat, supporting the observed cross-reactivity.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment (aa 33–259) covering the core serpin domain; expect recognition of both native and recombinant Antithrombin-III in WB and ELISA.
• For Western blotting, a band around 58 kDa is anticipated in reducing conditions; consider using plasma or liver lysates as positive controls.
• Cross-reactivity with mouse and rat has been listed; verify reactivity in the specific experimental system before quantitative assays.
• For ELISA, pairing with a detection antibody against a non-overlapping epitope may improve sensitivity; validate matrix effects using appropriate sample diluents.
• Avoid freeze-thaw cycles and store aliquots at -20°C or colder as recommended for polyclonal antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized provider of IVD raw materials, offering integrated sourcing support from early-stage research to commercial immunoassay development. This anti-SERPINC1 antibody addresses critical needs in coagulation diagnostics, and we can further supply validated matched antibody pairs, bulk ancillary reagents, and customized monoclonal or polyclonal antibodies for your application. Our team assists with rare target material procurement and technical consulting, ensuring a seamless supply chain for your diagnostic projects.