SERPINA10 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UK55

Core Product Specifications and Parameters

Biological Background: SERPINA10 Function and Localization

• SERPINA10 encodes the protein Z-dependent protease inhibitor (ZPI), also known as Serpin A10, a member of the serine protease inhibitor (serpin) superfamily.
• ZPI inhibits coagulation factor Xa in the presence of protein Z (PROZ), calcium, and phospholipids, playing a critical role in the regulation of blood coagulation.
• Additionally, ZPI can inhibit factor XIa independently of cofactors, broadening its anticoagulant spectrum.
• The protein is secreted from hepatocytes into the plasma, consistent with its role in systemic hemostasis.
• Post-translational modifications include glycosylation and phosphorylation; it also contains a heparin-binding domain that may modulate its activity.
• Keywords associated with SERPINA10 include blood coagulation, hemostasis, glycoprotein, heparin-binding, phosphoprotein, and secreted.

Experimental Guidance and Technical Tips

• The immunogen, comprising amino acids 165–444, targets a region outside the reactive center loop, potentially allowing detection of both free and complexed forms of ZPI.
• For WB, consider reducing-denaturing conditions given the predicted 51 kDa molecular weight; validate band specificity using relevant tissue or cell lysates.
• In IF/ICC, optimize fixation and permeabilization protocols, and include negative controls to confirm specificity for SERPINA10.
• For ELISA, evaluate performance in a sandwich format with appropriate capture/detection antibody pairs and calibrators.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Specializing in coagulation targets like SERPINA10, we support diagnostic manufacturers and research institutes from early development to clinical application. Contact us to leverage our streamlined supply chain and technical expertise.