Serotonin transporter Rabbit pAb for WB, IHC-P, ELISA - P31645

Core Product Specifications and Parameters

Biological Background: Serotonin transporter Function and Localization

• Sodium-dependent serotonin transporter (SERT), encoded by the SLC6A4 gene, belongs to the solute carrier family 6 and is also known as 5HTT or solute carrier family 6 member 4.
• SERT mediates high-affinity serotonin reuptake by cotransporting one serotonin molecule with one Na⁺ ion in exchange for one K⁺ ion (and possibly a proton) in an electroneutral cycle, terminating serotonergic signaling (PubMed:10407194, PubMed:12869649, PubMed:21730057). Related references: PMID:10407194 PMID:12869649 PMID:21730057
• The protein also exhibits an uncoupled, serotonin-induced channel-like conductance for monovalent cations (mainly Na⁺), which may influence membrane resting potential or excitability.
• In the central nervous system, SERT is essential for serotonin homeostasis; in the developing cortex, it controls serotonin uptake in glutamatergic neurons for proper spatial organization of cortical circuits.
• In the mature brain, SERT acts primarily in brainstem raphe neurons to clear synaptic serotonin.
• In the gastrointestinal tract, SERT modulates mucosal serotonin levels through uptake in enterocytes and is required for enteric neurogenesis and reflexes.
• In the blood, SERT ensures rapid, high-affinity serotonin uptake into platelets, regulating circulating serotonin levels (PubMed:17506858, PubMed:18317590). Related references: PMID:17506858 PMID:18317590
• Subcellularly, SERT localizes to the plasma membrane, endosome membrane, synaptic junctions, focal adhesions, and neuronal projections.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, as noted in UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–84) of human SERT; this design may be suitable for detecting total SERT protein. Validate specificity in your sample system.
• For Western blotting, a band around 70 kDa is expected. Membrane protein extraction protocols and gentle lysis conditions may improve detection.
• In IHC-P, antigen retrieval steps are often required; optimization of fixation and retrieval is advised.
• For ELISA, peptide competition assays can be used to confirm antibody specificity.
• Cross-reactivity with mouse and rat SERT has been observed; however, perform independent validation for non-human species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. For serotonin transporter research and assay development, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Partner with us to streamline your next neuroscience or serotonin signaling project.