SELENOK Rabbit pAb for WB and ELISA - Q9Y6D0

Core Product Specifications and Parameters

Biological Background: SELENOK Function and Localization

• SELENOK (Selenoprotein K), also designated SELK, HSPC030, or HSPC297, is a member of the selenoprotein family and incorporates selenocysteine in its sequence.
• It is critical for calcium (Ca²⁺) flux in immune cells, thereby promoting T-cell proliferation and neutrophil migration.
• SELENOK participates in endoplasmic reticulum-associated degradation (ERAD) of soluble glycosylated proteins. Related references: PMID:22016385
• Together with the palmitoyltransferase ZDHHC6, SELENOK mediates palmitoylation of inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) in immune cells, modulating ITPR1 stability and function. Related references: PMID:25368151
• The protein protects cells from endoplasmic reticulum stress-induced apoptosis. Related references: PMID:20692228
• When overexpressed in cardiomyocytes, SELENOK provides protection against oxidative stress damage. Related references: PMID:16962588
• SELENOK localizes predominantly to the endoplasmic reticulum membrane and the cell membrane.
• It is highly expressed in the heart and is involved in foam cell formation through CD36 palmitoylation and macrophage low-density lipoprotein uptake.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (amino acids 1-94) of human SELENOK; the resulting polyclonal antibody likely recognizes the full-length protein and possibly N-terminal fragments.
• For Western blotting, a band at approximately 11 kDa is expected; consider using a low-molecular-weight marker and optimizing transfer conditions for small proteins.
• Since the antibody cross-reacts with mouse SELENOK, it can be utilized in mouse-derived samples; however, validate in the relevant sample system due to potential species-specific differences.
• ELISA applications may benefit from peptide-based coating; consider determining optimal antibody dilution through checkerboard titration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing service for IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. With this SELENOK antibody, researchers focusing on selenoprotein biology, calcium signaling, or immune cell function can rely on CamelBio’s supply chain for consistent and scalable procurement. For rare-target requirements, custom antibody development and technical consultation are also available to accelerate your diagnostic projects from concept to clinic.