SCN2B Rabbit pAb for WB, ELISA - O60939

Core Product Specifications and Parameters

Biological Background: SCN2B Function and Localization

• SCN2B encodes the sodium channel regulatory subunit beta-2, an essential accessory protein that directly modulates the activity of multiple voltage-gated sodium (Nav) channels, including SCN1A/Nav1.1, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN8A/Nav1.6, SCN9A/Nav1.7, and SCN10A/Nav1.8. Related references: PMID:19808477 PMID:23559163 PMID:26894959
• Nav channels mediate membrane depolarization in excitable cells by selectively allowing Na+ ion influx, thereby initiating and propagating action potentials throughout neurons, cardiomyocytes, and skeletal muscle fibers. Related references: PMID:19808477 PMID:23559163 PMID:26894959
• The beta-2 subunit features an extracellular immunoglobulin (Ig) domain stabilized by a disulfide bond, contributing to both channel localization at the membrane and cell adhesion properties.
• SCN2B localizes predominantly to the cell membrane and is enriched in axonal projections, consistent with its critical role in neuronal action potential propagation.
• Mutations in SCN2B are clinically associated with cardiac arrhythmias such as atrial fibrillation and Brugada syndrome, underscoring its importance in cardiac electrophysiology.
• Post-translational modifications, including glycosylation and phosphorylation, are known to regulate SCN2B stability, trafficking, and functional modulation of Nav channels.
• The protein is synthesized as a precursor with an N-terminal signal peptide and contains a single transmembrane helix, anchoring it to the plasma membrane.

Experimental Guidance and Technical Tips

• Because the immunogen spans the extracellular domain (amino acids 30–160) of human SCN2B, the antibody may recognize both the full-length membrane-bound form and any proteolytically released soluble fragments; consider validating detection patterns in your specific sample types.
• For Western blot analysis, use reducing SDS-PAGE conditions and include a protein ladder with a marker near 24 kDa to account for the predicted molecular weight; verify the antibody’s specificity using human-derived neuronal or cardiac tissue lysates.
• When developing an ELISA, titrate the antibody against the immunogen or recombinant SCN2B protein to determine optimal coating and detection concentrations; include appropriate positive and negative controls to minimize background.
• As with any polyclonal antibody, batch-to-batch variability can occur — it is advisable to validate each new lot in your intended application before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including this SCN2B polyclonal antibody for ion channel studies. Our one-stop supply network covers validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate your diagnostic and research workflows. We also support rare target raw-material sourcing, helping you bridge from concept to clinic efficiently.