SCG3 Rabbit Polyclonal Antibody for WB and ELISA - Q8WXD2

Core Product Specifications and Parameters

Biological Background: SCG3 Function and Localization

• SCG3 is a member of the granin protein family and functions as a sorting receptor, regulating the biogenesis of secretory granules. Related references: PMID:19357184
• It specifically acts as a sorting receptor for chromogranin A (CHGA) and other intragranular proteins.
• SCG3 has been shown to promote angiogenesis by enhancing endothelial cell proliferation, migration, and tube formation via the MEK/ERK signaling pathway. Related references: PMID:29154827
• The protein localizes to cytoplasmic vesicles and secretory vesicle membranes, and is also secreted.
• SCG3 is expressed in multiple tissues, including brain, heart, kidney, liver, and skeletal muscle, and has been detected in urine. Related references: PMID:25326458
• Post-translational modifications include cleavage on dibasic sites, glycosylation, phosphorylation, and proteoglycan attachment.
• Alternative splicing generates multiple isoforms of SCG3.
• It belongs to the reference proteome and has been identified in proteomic studies.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 199-468 of human SCG3, which may confer specificity for detecting the target in various applications.
• In Western blot, SCG3 migrates at an expected molecular weight of 53 kDa, but post-translational modifications may lead to band shifts; verify with appropriate controls.
• This antibody is suitable for ELISA-based quantification; however, the optimal working dilution should be determined empirically.
• The reported cross-reactivity with mouse and rat suggests utility in rodent model studies; confirm reactivity in your system.
• As a polyclonal antibody, lot-to-lot consistency may vary; consider validating each new lot in a pilot experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined sourcing platform for high-quality IVD raw materials. This SCG3 polyclonal antibody supports secretory granule biology and angiogenesis research, and is part of our comprehensive portfolio that includes validated antibody pairs, recombinant proteins, and ancillary reagents. Whether you need bulk quantities or rare target sourcing, CamelBio provides reliable end-to-end supply solutions.