SASH1 Rabbit Polyclonal Antibody for WB and ELISA - O94885

Core Product Specifications and Parameters

Biological Background: SASH1 Function and Localization

• SASH1, also known as SAM and SH3 domain-containing protein 1, acts as a scaffold molecule downstream of TLR4 activation, positively regulating NF-κB signaling by assembling a complex that includes TRAF6, MAP3K7, CHUK, and IKBKB. Related references: PMID:23776175
• It regulates the ubiquitination of TRAF6 and MAP3K7, facilitating signal transduction. Related references: PMID:23776175
• SASH1 is involved in the regulation of cell mobility, including endothelial cell migration in response to lipopolysaccharide (LPS) and melanocyte migration during skin pigmentation. Related references: PMID:23333244, PMID:23776175, PMID:25315659
• The protein contains SH3 and SAM domains and is classified as a tumor suppressor; it is down-regulated in a majority of breast tumors.
• Subcellularly, SASH1 localizes predominantly to the cytoplasm.
• SASH1 is ubiquitously expressed with highest levels in lung, placenta, spleen, and thymus; in the skin, it is found in epidermal keratinocytes, dermal fibroblasts, and melanocytes. Related references: PMID:23333244, PMID:26203640
• Post-translational modifications include phosphorylation, and disease variants are associated with hypotrichosis and palmoplantar keratoderma.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-300); consider using peptide blocking or truncated constructs to verify epitope specificity in your experiments.
• For Western blotting, a band near 137 kDa is expected; however, post-translational modifications or isoform differences may cause shifts, so validate in your relevant sample system.
• The antibody cross-reacts with mouse SASH1, supporting its use in mouse models; confirm cross-reactivity and optimize conditions for your specific assays.
• For ELISA, consider using recombinant SASH1 protein or cell lysates as coating antigens, and include appropriate negative controls (e.g., SASH1 knockdown samples) to assess background.
• As a polyclonal antibody, lot-to-lot consistency should be verified, and inclusion of a blocking peptide or knockout validation is recommended to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers, labs, and research institutes with a comprehensive one-stop pipeline for IVD raw materials, technical services, and consulting—from concept to clinic. For SASH1-related research in NF-κB signaling, cell migration, and tumor suppression, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets. Partner with CamelBio to streamline your procurement and accelerate assay development with reliable, research-grade materials.