SARS-CoV-2 ORF9b Rabbit Polyclonal Antibody for WB, ELISA - P0DTD2

Core Product Specifications and Parameters

Biological Background: SARS-CoV-2 ORF9b Function and Localization

• The SARS-CoV-2 ORF9b protein is an accessory protein that plays a key role in viral immune evasion by inhibiting the host innate immune response.
• It localizes to the host cytoplasm and mitochondria, where it interferes with mitochondrial-associated antiviral signaling.
• ORF9b directly binds to host TOMM70, preventing its interaction with HSP90AB1, which disrupts the interferon activation pathway.
• This protein is involved in the inhibition of host MAVS and RLR pathways, critical for antiviral defense.
• According to UniProt, ORF9b is a reference proteome component with a calculated molecular weight of approximately 10.8 kDa.
• The protein has been structurally characterized (3D-structure available) and is annotated with keywords including host-virus interaction and viral immunoevasion.

Experimental Guidance and Technical Tips

• For Western blot, use a low molecular weight marker to accurately monitor the 11 kDa ORF9b band; consider a 15% SDS-PAGE gel for optimal resolution.
• Validate the antibody's specificity by comparing signals in infected versus uninfected cell lysates or using a negative control (e.g., unrelated viral protein).
• In ELISA applications, the polyclonal nature may provide high sensitivity; however, cross-reactivity with closely related coronaviral proteins should be assessed.
• Due to the mitochondrial localization of ORF9b, subcellular fractionation may be employed to enrich the target and improve detection in complex samples.
• Store the antibody as recommended (ice bag shipping indicates cold-chain requirement) and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with reliable access to high-quality IVD raw materials, including validated antibody pairs and bulk reagents. This anti-SARS-CoV-2 ORF9b antibody supports COVID-19 research and assay development, complementing our portfolio of infectious disease targets. For rare or custom targets, our sourcing network can secure specialized raw materials to accelerate your project from concept to clinic.