SARS-CoV-2 ORF3A Rabbit pAb - P0DTC3

Core Product Specifications and Parameters

Biological Background: SARS-CoV-2 ORF3A Protein Function and Localization

• The ORF3a protein is an accessory protein of SARS-CoV-2, also known as Protein 3a, Protein U274, or Protein X1.
• It plays a key role in viral egress by facilitating lysosomal trafficking of virions (PubMed:33157038, PubMed:33422265). Related references: PMID:33157038 PMID:33422265
• ORF3a forms homotetrameric ion channels (viroporins) in endosomal and lysosomal membranes, potentially inducing lysosomal deacidification to promote safe viral release (PubMed:33157038, PubMed:33422265, PubMed:34158638). Related references: PMID:33157038 PMID:33422265 PMID:34158638
• The protein disrupts host autophagy by binding VPS39 on late endosomes, which blocks autophagosome-lysosome fusion and autolysosome formation (PubMed:33422265). Related references: PMID:33422265
• ORF3a induces RETREG1/FAM134B-dependent reticulophagy by interacting with HMGB1, enhancing HMGB1-BECN1 association and causing ER stress and pro-inflammatory responses (PubMed:35239449). Related references: PMID:35239449
• Subcellularly, ORF3a localizes to the virion, host cell membrane, endoplasmic reticulum membrane, secreted forms, cytoplasm, endosomes, and lysosomes.
• Key biological keywords include glycoprotein, host-virus interaction, ion channel, ion transport, activation of host autophagy, and viroporin activity.
• The protein has a calculated molecular weight of ~31.1 kDa (UniProt), consistent with the observed 31 kDa expected for this antibody.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100-200 of the ORF3a protein; antibody binding epitopes may be concentrated in this region, which should be considered when designing experiments or interpreting results.
• For Western blotting, the expected band for ORF3a is around 31 kDa; validate specificity using lysates from SARS-CoV-2 infected cells or ORF3a-overexpressing systems.
• In ELISA applications, confirm optimal coating conditions and antibody dilution using recombinant ORF3a protein or defined positive controls.
• As a polyclonal antibody, lot-to-lot consistency may vary; it is advisable to qualify each new batch in the intended application prior to large-scale use.
• The antibody is shipped on wet ice; upon receipt, aliquot and store at -20°C (or as recommended by the manufacturer) to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing platform for IVD raw materials, offering diagnostic manufacturers and laboratories streamlined access to high-quality reagents such as this SARS-CoV-2 ORF3a antibody. We supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare target materials for infectious disease research and diagnostic development. Count on our integrated procurement support to accelerate your virology projects from concept to clinic.