SAP18 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O00422

Core Product Specifications and Parameters

Biological Background: SAP18 Function and Localization

• SAP18, also known as Sin3-associated polypeptide p18 or 2HOR0202, is a component of the SIN3-repressing complex, enhancing SIN3-HDAC1-mediated transcriptional repression by directing repressive complexes to core histone proteins.
• As an auxiliary component of the exon junction complex (EJC), SAP18 is deposited at splice junctions on mRNAs and participates in mRNA metabolism and nonsense-mediated decay.
• SAP18 is part of the ASAP and PSAP complexes, which bind RNA sequence-independently, regulate intron excision, and inhibit mRNA processing; these complexes promote apoptosis and disassemble upon apoptotic induction.
• It modulates alternative splicing of apoptotic genes, specifically inhibiting formation of the proapoptotic Bcl-X(S) isoform, thereby linking transcription regulation to apoptosis.
• SAP18 localizes to the nucleus, cytoplasm, and nuclear speckles, consistent with its dual roles in transcription and mRNA processing.
• Ubiquitously expressed, SAP18 is involved in core cellular processes including transcription regulation, mRNA splicing, and apoptosis.
• The protein is subject to acetylation and alternative initiation, with a molecular weight of approximately 18 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 21–172 of human SAP18, a region critical for its functional interactions; consider this when designing blocking or competition experiments.
• For Western blotting, expect a band near 18 kDa; nuclear or whole-cell extracts are recommended. Validate antibody performance in relevant sample systems.
• In IHC-P, optimization of antigen retrieval and primary antibody dilution is advised; staining may be observed in nuclear and cytoplasmic compartments.
• For ELISA, the antibody can be used to detect SAP18 in cell lysates or purified protein; cross-reactivity with mouse and rat SAP18 should be confirmed experimentally.
• Cross-reactivity with mouse and rat is expected based on sequence homology, but independent validation in each species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This SAP18 antibody supports research into transcriptional regulation, splicing, and apoptosis. Through CamelBio, you can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials to streamline your assay development from concept to clinic.