SAMM50 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q9Y512

Core Product Specifications and Parameters

Biological Background: SAMM50 Function and Localization

• SAMM50, also known as Sorting and assembly machinery component 50 homolog or Transformation-related gene 3 protein (TRG-3), is a key mitochondrial outer membrane protein encoded by the SAMM50 gene.
• It plays a crucial role in maintaining mitochondrial cristae structure and ensuring proper assembly of the mitochondrial respiratory chain complexes. Related references: PMID:22252321 PMID:25781180
• SAMM50 is also required for the assembly of TOMM40 into the TOM complex, facilitating mitochondrial protein import. Related references: PMID:15644312
• The protein localizes to the mitochondrial outer membrane, with additional presence in the cytoplasm and general mitochondrion.
• SAMM50 is classified as a transmembrane beta-strand protein, highlighting its structural integration into the mitochondrial outer membrane.
• Post-translational modifications include methylation, and it has been identified through proteomics studies.
• Its molecular weight is approximately 52 kDa, consistent with its role as a major component of the sorting and assembly machinery.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to the N-terminal region (amino acids 1–220) of human SAMM50, which may provide epitopes that are accessible in both native and denatured conditions.
• For Western blotting, the predicted band is ~52 kDa; consider using appropriate positive controls such as mitochondrial fractions from human, mouse, or rat cells.
• In immunohistochemistry (IHC-P) and immunoprecipitation (IP), validate the antibody in the relevant sample system and optimize fixation/permeabilization protocols as needed.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; including known positive and negative controls in each experiment is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of monoclonal and polyclonal antibodies, recombinant proteins, and ancillary reagents. The SAMM50 rabbit polyclonal antibody is ideal for mitochondrial function and respiratory chain assembly studies, and we can support your projects with bulk supply, custom antibody development, and technical consultation. Our streamlined procurement process ensures timely delivery and consistent quality for diagnostic and research applications.