SAFB Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q15424

Core Product Specifications and Parameters

Biological Background: SAFB Function and Localization

• SAFB (scaffold attachment factor B1), also designated HAP, HET, SAF-B, or SAFB1, binds to scaffold/matrix attachment region (S/MAR) DNA and serves as a molecular assembly point for a 'transcriptosomal' complex coupling transcription with RNA processing. Related references: PMID:9671816
• SAFB functions as an estrogen receptor corepressor; it binds the HSP27 promoter and reduces its transcription, thus acting as a negative regulator of cell proliferation. Related references: PMID:12660241
• When associated with RBMX, SAFB binds to and stimulates transcription from the SREBF1 promoter (By similarity).
• Subcellular localization is restricted to the nucleus.
• Ubiquitously expressed, with high levels in the central nervous system and low levels in the liver; also found across a wide range of breast cancer cell lines.
• Undergoes multiple post‑translational modifications, including acetylation, methylation, phosphorylation, and ubiquitin‑like conjugation (isopeptide bond), as indicated by UniProt keywords.
• Categorized as a DNA‑binding, RNA‑binding transcription repressor; alternative splicing yields multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen covers the N‑terminal region (aa 1–200) of human SAFB; this region is largely conserved in mouse and rat, consistent with observed cross‑reactivity.
• For Western blot, a band near 103 kDa is expected; tissue or cell lines with high SAFB expression (e.g., CNS‑derived or breast cancer lines) may serve as positive controls.
• In IHC‑P and IF/ICC, nuclear staining patterns should be observed; validate in the relevant sample system and include appropriate controls.
• ELISA suitability suggests the antibody can detect native or denatured SAFB antigen; titration under the specific assay conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw‑material procurement by offering validated antibodies, bulk ancillary reagents, and custom sourcing for rare targets—all through a single partner. This SAFB antibody complements our portfolio for transcription regulation and chromatin biology research, and we can supply matched antibody pairs, optimized monoclonals, or technical consulting to support your assay development from concept to clinic.