SAA4 Rabbit Polyclonal Antibody for WB, ELISA - P35542

Core Product Specifications and Parameters

Biological Background: SAA4 Function and Localization

• Serum amyloid A-4 protein (SAA4), also known as constitutively expressed serum amyloid A protein (C-SAA), is a member of the serum amyloid A family of apolipoproteins associated with high-density lipoprotein (HDL).
• SAA4 functions as a major acute phase reactant, with its plasma concentration increasing in response to inflammation, infection, or tissue injury.
• The protein is secreted and contains a signal peptide, consistent with its extracellular role in lipid transport and immune modulation.
• SAA4 is predominantly expressed by the liver and secreted into the bloodstream, serving as a systemic marker of the acute phase response.
• As a glycoprotein, SAA4 undergoes post-translational glycosylation, which may influence its stability, HDL binding, or functional interactions.
• Its classification as a reference proteome component underscores its well-characterized sequence and importance in biomedical research.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to a synthetic peptide from amino acid 50 to the C-terminus of human SAA4; consider sequence alignment to assess cross-reactivity with other SAA family members or isoforms.
• For Western blotting, given the predicted protein weight of approximately 15 kDa, optimize gel percentage (e.g., 12–15% SDS-PAGE) and transfer conditions to ensure clear resolution of low-molecular-weight bands.
• The antibody is reactive with human, mouse, and rat samples, making it suitable for comparative studies; however, always validate performance in the relevant species and experimental system.
• For ELISA applications, titrate the antibody concentration and confirm binding specificity using recombinant SAA4 or known positive/negative samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, connecting diagnostic manufacturers, labs, and research institutes with reliable supplies from concept to clinic. For acute phase and inflammation research areas such as SAA4, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing support for rare-target raw materials. Let CamelBio streamline your procurement with a full spectrum of high-quality immunoreagents tailored to your assay development needs.