S100B Rabbit mAb for WB, IF, IHC-P, ELISA - P04271

Core Product Specifications and Parameters

Biological Background: S100B Function and Localization

• Small zinc- and calcium-binding protein highly expressed in astrocytes; constitutes one of the most abundant soluble proteins in brain. Binds zinc tightly and calcium weakly, with distinct binding sites for both ions on each monomer. Related references: PMID:20950652 PMID:6487634
• Functions as a neurotrophic factor, promoting astrocytosis and axonal proliferation; also acts as an adipocyte-derived neurotrophic factor to promote sympathetic innervation of thermogenic adipose tissue.
• Binds to and activates STK38 by releasing autoinhibitory intramolecular interactions; interaction with AGER after myocardial infarction may play a role in myocyte apoptosis via ERK1/2 and p53/TP53 signaling.
• Assists ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. Related references: PMID:20351179
• Mediates calcium-dependent regulation of physiological processes by interacting with TPR-containing proteins and modulating their activity. Related references: PMID:22399290
• Subcellular localization includes cytoplasm, nucleus, and secretion; also found in a variety of tissues beyond brain, though predominantly a water-soluble brain protein.
• Keywords: 3D-structure, Acetylation, Calcium, Cell adhesion, Cytoplasm, Metal-binding, Nucleus, Repeat, Secreted, Zinc.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human S100B protein (aa 1–92). The antibody may detect both monomeric and dimeric forms; perform reducing and non-reducing SDS-PAGE to characterize band patterns.
• For Western blotting, use high-percentage gels (e.g., 15%) to resolve the ~11 kDa S100B monomer. Transfer to PVDF membrane with 0.22 µm pore size for high retention of low-molecular-weight proteins.
• In immunostaining applications, S100B can be present in cytoplasm, nucleus, and extracellular space. Include appropriate permeabilization steps for intracellular detection and consider serum-free blocking to minimize background from secreted protein.
• Cross-reactivity is reported for human, mouse, and rat; validate in the relevant sample system, as recognition may depend on sequence conservation and post-translational modifications.
• For ELISA, this antibody can be explored as a capture or detection reagent in a matched pair; titrate and optimize against the recombinant immunogen or native protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material procurement, connecting diagnostic manufacturers, reference labs, and research institutes with high-quality reagents and technical expertise. This anti-S100B antibody is ideally suited for neuroscience, oncology, and neurodegenerative disease assay development—where reliable detection of astrocyte-derived S100B is critical. Beyond a single antibody, CamelBio sources validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and even rare-target raw materials, ensuring your projects progress seamlessly from concept to clinic.