S100B Mouse mAb for WB - P04271

Core Product Specifications and Parameters

Biological Background: S100B Function and Localization

• S100B (S-100 protein beta chain, S100 calcium-binding protein B) is a small, abundant calcium- and zinc-binding protein highly expressed in astrocytes, constituting one of the most abundant soluble proteins in brain.
  Related references: PMID:20950652 PMID:6487634
• It weakly binds calcium but tightly binds zinc, with distinct binding sites for each ion on each monomer; potassium antagonizes binding of both divalent cations, especially at high-affinity calcium-binding sites.
  Related references: PMID:20950652 PMID:6487634
• Acts as a neurotrophic factor promoting astrocytosis and axonal proliferation, and is involved in the innervation of thermogenic adipose tissue by promoting sympathetic innervation.
• Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization.
  Related references: PMID:20351179
• May mediate calcium-dependent regulation of physiological processes by interacting with TPR-containing proteins and modulating their activity.
  Related references: PMID:22399290
• Localizes to the cytoplasm and nucleus, and is also secreted.
• Although predominantly a brain protein, S100B is also found in a variety of other tissues.
• Undergoes acetylation post-translational modification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human S100B protein (amino acids 1-92), suggesting the antibody may recognize the native conformation and linear epitopes. Consider validating in applications requiring native structure detection.
• For Western blot (WB), the predicted molecular weight is approximately 11 kDa; a band near this size may be observed in brain or other S100B-expressing tissues.
• This antibody is listed for immunofluorescence (IF-P), immunohistochemistry (IHC-P), and ELISA; optimization of dilution factors and antigen retrieval conditions is recommended based on specific sample types.
• Cross-reactivity with mouse and rat S100B is stated, making it potentially suitable for studies using rodent models; confirm species reactivity in the relevant experimental system.

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CamelBio is your one-stop IVD raw-material sourcing partner, providing comprehensive access to validated antibodies, bulk ancillary reagents, and rare target procurement. This S100B mouse monoclonal antibody supports neuroscience and biomarker research applications. Let us help streamline your project from concept to clinic.