S100A5 Rabbit pAb - P33763

Core Product Specifications and Parameters

Biological Background: S100A5 Function and Localization

• Protein S100-A5, also known as protein S-100D or S100 calcium-binding protein A5, is encoded by the S100A5 gene.
• It belongs to the S100 family of small, acidic calcium-binding proteins characterized by EF-hand motifs.
• Functionally, S100A5 binds calcium, zinc, and copper ions. One subunit can simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion, with calcium and copper competing for the same binding sites.
• The metal-binding properties suggest involvement in intracellular signaling, metal ion homeostasis, and possibly antimicrobial responses.
• Structural features include alternative splicing and a known 3D structure (per UniProt keywords), indicating a well-characterized protein.
• As a reference proteome component, S100A5 serves as a model for studying S100 protein family functions.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-92 of human S100A5 (NP_002953.2), which corresponds to the full-length protein. As a polyclonal antibody, it may recognize multiple epitopes, enhancing detection sensitivity in various assays.
• For Western blotting (WB), consider using reducing conditions and expect a band near 11 kDa. Validate the antibody’s specificity using relevant positive (e.g., S100A5-expressing human cells) and negative controls.
• In immunohistochemistry-paraffin (IHC-P), optimize antigen retrieval and antibody dilution for your tissue type. Since cross-reactivity is reported only for human, confirm performance in human samples; reactivity in other species is not characterized.
• For ELISA, pair this antibody with a matched detection reagent (e.g., a validated monoclonal anti-S100A5) for sandwich ELISA development. Consider testing a range of concentrations to establish optimal signal-to-noise ratios.
• Note that this antibody has not been validated for applications beyond those listed; for other uses, pilot experiments are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined, one-stop sourcing solution for IVD raw materials, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. The S100A5 rabbit polyclonal antibody addresses research interests in calcium signaling, metal ion biology, and potential biomarker studies. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and expertise in sourcing rare target raw materials to accelerate your assay development. Contact us to discuss how we can support your specific project needs.