S100A10 Rabbit pAb - P60903

Core Product Specifications and Parameters

Biological Background: S100A10 Function and Localization

• S100A10, also known as p11, calpactin I light chain, or ANX2LG, is a member of the S100 family of EF-hand calcium-binding proteins.
• It typically forms a heterotetramer with annexin A2 (ANXA2), where two S100A10 subunits bind two ANXA2 molecules.
• S100A10 induces dimerization of ANXA2, and the ANXA2 monomer is the preferred target of tyrosine-specific kinase, suggesting a role in regulating protein phosphorylation.
• The protein undergoes post-translational modifications including acetylation and isopeptide bond formation (Ubl conjugation), as identified by proteomics.
• Its molecular weight is approximately 11 kDa.
• UniProt keywords for S100A10 include 3D-structure, acetylation, isopeptide bond, proteomics identification, reference proteome, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human S100A10 protein (amino acids 1-97), so the antibody may detect both native and denatured forms; consider appropriate sample preparation and controls.
• For WB, a band at approximately 11 kDa is expected; validate detection in the relevant species and tissue lysates.
• For IHC-P, test antigen retrieval and antibody dilution on FFPE tissue sections from human, mouse, rat, or monkey samples.
• For IF/ICC, optimize fixation and permeabilization conditions for the target cell type to preserve S100A10 localization.
• Cross-reactivity is reported for human, mouse, rat, and monkey; however, validation in your specific model system is recommended.

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For researchers and diagnostic developers studying calcium signaling or protein phosphorylation, CamelBio offers a comprehensive sourcing solution for IVD raw materials. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare-target materials to support your assay development from concept to clinic.