RYBP Rabbit Polyclonal Antibody for WB, ELISA - Q8N488

Core Product Specifications and Parameters

Biological Background: RYBP Function and Localization

• RYBP (RING1 and YY1-binding protein), also known as AAP1, DEDAF, YEAF1, and APAP-1, is a Polycomb group (PcG) protein that functions as a core component of the PRC1-like multiprotein complex.
• As part of the PRC1-like complex, RYBP mediates monoubiquitination of histone H2A at 'Lys-119', contributing to chromatin remodeling and transcriptional repression of target genes, including Hox genes. Related references: PMID:25519132
• RYBP inhibits ubiquitination and subsequent degradation of TP53, thereby regulating transcription of TP53 target genes and playing a role in DNA damage response. Related references: PMID:19098711
• It may regulate ubiquitin-mediated proteasomal degradation of other proteins, such as FANK1, to modulate apoptosis. Related references: PMID:14765135, PMID:27060496
• RYBP functions as a transcriptional repressor of E4TF1 and may also bind DNA directly. Related references: PMID:11953439
• In breast cancer, RYBP is down-regulated and acts as a tumor suppressor by down-regulating SRRM3, reducing growth and metastasis. Related references: PMID:27748911
• Subcellularly, RYBP localizes to the nucleoplasm, nucleus, and cytoplasm, and is widely expressed with highest levels in lymphoid tissues and placenta.
• The protein contains zinc finger domains, undergoes phosphorylation, and is subject to ubiquitination-like modifications (isopeptide bond formation).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the C-terminal region (amino acids 100 to C-terminus) of human RYBP; consider using this peptide as a positive control or coating antigen in ELISA.
• For Western Blot (WB), expect a band at ~25 kDa in whole-cell lysates from human, mouse, or rat cells/tissues; validate with appropriate negative and positive controls in your specific sample system.
• Cross-reactivity with mouse and rat has been observed, but performance in other species or applications should be verified experimentally.
• Optimize antibody dilution for your assay conditions; typical starting dilutions for WB are 1:500–1:2000 and for ELISA 1:1000–1:5000, but these should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This RYBP polyclonal antibody is suited for epigenetics, cancer, and apoptosis research, particularly in Western Blot and ELISA applications. As your procurement partner, CamelBio can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials to streamline assay development and clinical translation.