RUNX1/2/3 Rabbit Monoclonal Antibody for WB, ELISA - Q01196 / Q13761 / Q13950

Core Product Specifications and Parameters

Biological Background: RUNX1/2/3 Function and Localization

• The RUNX family of transcription factors includes RUNX1 (Gene ID 861), RUNX2 (864), and RUNX3 (860), which share a conserved Runt domain. This antibody is raised against a synthetic peptide from amino acids 354-453 of human RUNX1/2/3, enabling recognition of all three members.
• RUNX1 (Runt-related transcription factor 1), also known as AML1 or CBF-alpha-2, forms a heterodimeric core-binding factor (CBF) complex with CBFB to regulate gene expression.
• It binds to the core consensus sequence 5'-TGTGGT-3' via its Runt domain and is essential for normal hematopoietic development (PubMed:17431401).
  Related references:
  PMID:17431401
• RUNX1 acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter (PubMed:10207087, PubMed:14970218).
  Related references:
  PMID:10207087 PMID:14970218
• It plays a critical role in T-cell development and regulatory T-cell function, where it associates with FOXP3 to control anergy and suppressive activity (PubMed:17377532).
  Related references:
  PMID:17377532
• The protein is localized to the nucleus and is expressed in most tissues except brain and heart, with highest levels in thymus, bone marrow, and peripheral blood.
• Post-translational modifications include acetylation, phosphorylation, and methylation, as indicated by keywords such as Acetylation, Phosphoprotein, and Methylation.

Experimental Guidance and Technical Tips

• For Western blot, consider optimizing detection conditions for a band at approximately 51 kDa in human or mouse samples. Use appropriate loading controls and validate specificity for the intended RUNX isoform.
• The immunogen sequence is conserved among RUNX1, RUNX2, and RUNX3; cross-validation with isoform-specific controls or knockdown/knockout models may be necessary to confirm target specificity.
• For ELISA, consider using the antibody as a capture or detection reagent with known positive controls and calibrate with recombinant RUNX proteins if available.
• Shipping on ice bags ensures product stability; aliquot and store according to laboratory guidelines to maintain long-term activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined one-stop sourcing solution for IVD raw materials, technical services, and consulting from concept to clinic. For researchers targeting hematopoietic transcription factors and leukemia biomarkers, we offer validated monoclonal antibodies like this RUNX1/2/3 Rabbit mAb, along with complementary reagents, bulk supplies, and custom sourcing for rare targets. Our platform simplifies procurement, enabling you to accelerate your assay development with confidence.