RUNX1 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q01196

Core Product Specifications and Parameters

Biological Background: RUNX1 Function and Localization

• RUNX1, also known as AML1 or CBFA2, is a transcription factor that forms a heterodimeric complex with CBFB to regulate gene expression.
• It binds to the core consensus DNA sequence 5'-TGTGGT-3' via its runt domain, with CBFB enhancing DNA-binding affinity allosterically.
• Essential for normal hematopoiesis, as demonstrated by its role in developmental processes. Related references: PMID:17431401
• RUNX1 synergizes with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter. Related references: PMID:10207087, PMID:14970218
• It plays a critical role in T-cell development, including repressing ZBTB7B to allow cytotoxic T-cell differentiation and controlling regulatory T-cell function by associating with FOXP3. Related references: PMID:17377532
• Localized predominantly in the nucleus, RUNX1 is expressed in all tissues except brain and heart, with highest levels in thymus, bone marrow and peripheral blood.
• Post-translational modifications such as acetylation, phosphorylation, and methylation regulate its activity, and RUNX1 is implicated in chromosomal rearrangements associated with leukemia (keywords: proto-oncogene, disease variant).

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a peptide from the central region of RUNX1. Validate specificity using knockout or knockdown models.
• For Western blot, expect a band around 49 kDa; post-translational modifications may cause slight shifts.
• In immunocytochemistry and immunohistochemistry, expect nuclear localization; optimize fixation and permeabilization conditions.
• The antibody may recognize multiple RUNX1 isoforms; confirm reactivity in your experimental system.
• Use positive controls such as thymus or bone marrow lysates, where RUNX1 expression is high.
• For ELISA, titrate the antibody and use appropriate blocking to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. This anti-RUNX1 polyclonal antibody is ideal for studying transcription regulation in hematopoiesis and leukemia; we also offer validated antibody pairs, bulk ancillary reagents, and sourcing for rare targets to streamline your assay development.