RPS20 Rabbit pAb for WB, IF/ICC, ELISA - P60866

Core Product Specifications and Parameters

Biological Background: RPS20 Function and Localization

• RPS20, also known as uS10 or 40S ribosomal protein S20, is a core component of the small (40S) ribosomal subunit.
  Related references:
  PMID:23636399
• As part of the ribosome, the large ribonucleoprotein complex, RPS20 participates in the initiation and elongation phases of mRNA translation, ensuring accurate protein synthesis.
• RPS20 localizes predominantly to the cytoplasm, consistent with its ribosomal function, though it may transiently be found in the nucleus during ribosome assembly.
• The protein is subject to post-translational modifications including acetylation, phosphorylation, and ubiquitin conjugation, which may regulate ribosome activity or protein stability.
• High-resolution structural studies have mapped RPS20 within the 40S subunit, revealing its critical role in maintaining the decoding site architecture.
• RPS20 is widely expressed across tissues and is conserved across eukaryotes, reflecting its essential housekeeping role.

Experimental Guidance and Technical Tips

• For Western blotting, a band at approximately 13 kDa corresponding to endogenous RPS20 is expected in human, mouse, and rat cell lysates. Optimize loading and transfer conditions for low-molecular-weight proteins.
• For immunofluorescence/immunocytochemistry, fix cells with 4% paraformaldehyde and permeabilize with 0.1% Triton X-100. Validate staining specificity using RPS20 knockdown or knockout controls.
• In ELISA applications, consider using the recombinant immunogen as a positive control to establish assay linearity and sensitivity.
• The polyclonal nature of the antibody may detect multiple isoforms or modification states; verify banding patterns in relevant biological contexts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research laboratories with one-stop access to high-quality raw materials, including validated antibodies like this RPS20 polyclonal antibody for ribosomal research. Beyond standard catalog products, we offer customized sourcing of optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials to streamline your diagnostic development pipeline. Partner with CamelBio to accelerate your project from concept to clinic with reliable, technical supply support.