RPS15A Rabbit pAb for WB, IHC-P, ELISA - P62244

Core Product Specifications and Parameters

Biological Background: RPS15A Function and Localization

• RPS15A (40S ribosomal protein S15a, also known as uS8, S15a, DBA20) is a component of the small 40S ribosomal subunit. Related references: PMID:23636399
• During ribosome biogenesis, RPS15A is incorporated into the small subunit (SSU) processome in the nucleolus, where it assists in pre-rRNA folding, modification, and cleavage, ultimately contributing to mature 40S subunit assembly. Related references: PMID:34516797
• RPS15A is essential for erythropoiesis, as mutations are associated with Diamond-Blackfan anemia, a congenital red cell aplasia. Related references: PMID:27909223
• The protein localizes predominantly to the cytoplasm, nucleus, and nucleolus, consistent with its dual roles in ribosome assembly and function.
• RPS15A belongs to the universal ribosomal protein uS8 family, highly conserved across eukaryotes.
• The canonical isoform has a predicted molecular weight of ~14.8 kDa (UniProt), consistent with the ~15 kDa observed protein weight.
• Keywords: 3D-structure, Diamond-Blackfan anemia, direct protein sequencing, proteomics identification, ribonucleoprotein, ribosomal protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human RPS15A (amino acids 1–130), ensuring recognition of the native protein in multiple species due to high sequence conservation; consider sequence alignment for other species.
• For Western blot, a band at ~15 kDa is expected; validate the antibody with appropriate positive and negative controls, such as lysates from RPS15A-expressing tissues (e.g., liver, spleen).
• In immunohistochemistry (paraffin), antigen retrieval conditions may need optimization; nuclear and cytoplasmic staining patterns are anticipated.
• For ELISA, the antibody can be used as a capture or detection reagent; pair with a matched monoclonal antibody for sandwich assays if desired.
• Cross-reactivity with mouse and rat suggests suitability for translational models; always verify in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories with a full range of high-quality antibodies, including polyclonal and monoclonal options against ribosomal targets like RPS15A. We offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your assay development from concept to clinic. Our expertise in ribosome biology and related disorders ensures reliable supply solutions for your projects.