RPL7 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P18124

Core Product Specifications and Parameters

Biological Background: RPL7 Function and Localization

• RPL7 (60S ribosomal protein L7, also known as uL30) is a component of the large ribosomal subunit, essential for protein synthesis. Related references: PMID:12962325 PMID:23636399 PMID:32669547
• The protein binds to G-rich structures in 28S rRNA and in mRNAs, contributing to ribosome assembly and translation. Related references: PMID:12962325
• RPL7 plays a regulatory role in translation; it inhibits cell-free translation of mRNAs, suggesting a modulatory function. Related references: PMID:12962325
• Subcellular localization: RPL7 is predominantly found in the cytoplasm, consistent with its role in translation.
• Post-translational modifications of RPL7 include acetylation and phosphorylation, which may regulate protein interactions and ribosome function.
• RPL7 belongs to the universal ribosomal protein uL30 family and contains repeat regions, classified as a ribonucleoprotein and RNA-binding protein.

Experimental Guidance and Technical Tips

• Because the immunogen corresponds to the full-length human RPL7 protein, the antibody may detect the target across a range of applications including WB, IHC, IF, and ELISA. Consider validating the antibody in your specific sample system and application conditions.
• The predicted molecular weight of RPL7 is ~29 kDa (UniProt: 29.2 kDa); in WB, a band near this size is expected, though post-translational modifications may cause slight shifts.
• For IHC-P, antigen retrieval and antibody dilution may need optimization; test with known positive control tissues (e.g., highly proliferative tissues with abundant ribosomes).
• Cross-reactivity is reported for human, mouse, and rat; if using in other species, verify sequence homology of the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

Looking for reliable IVD raw materials for your immunoassay development? CamelBio serves as your integrated sourcing bridge, offering a comprehensive portfolio of validated antibodies (including this anti-RPL7 polyclonal antibody) and ancillary reagents for ribosomal protein studies and translation research. From bulk monoclonal/polyclonal antibody supply to rare target sourcing, we support diagnostic manufacturers and research labs with one-stop procurement solutions.