RPL35A Rabbit Polyclonal Antibody for WB and ELISA - P18077

Core Product Specifications and Parameters

Biological Background: RPL35A Function and Localization

• Component of the large ribosomal subunit: RPL35A (eL33) is an integral component of the 60S ribosomal subunit, which together with the 40S subunit forms the 80S ribosome responsible for cellular protein synthesis.

Related references: PMID:23636399, PMID:32669547

• Protein synthesis core function: The ribosome decodes mRNA into polypeptide chains via peptidyl transferase activity, a process essential for cell growth and maintenance.

Related references: PMID:23636399, PMID:32669547

• Hematopoietic cell proliferation: RPL35A is specifically required for the proliferation and viability of hematopoietic cells, linking it to erythropoiesis and bone marrow function.

Related references: PMID:18535205

• Disease association: Mutations in RPL35A are associated with Diamond-Blackfan anemia (DBA), a congenital erythroid aplasia characterized by defective ribosome biogenesis in hematopoietic progenitors.

• Subcellular localization: RPL35A localizes predominantly in the cytoplasm, where it participates in ribosomal assembly and translation.

• Post-translational modifications and interactions: The protein is subject to acetylation and is classified as a ribonucleoprotein with RNA-binding and tRNA-binding capabilities, highlighting its role in the ribosomal machinery.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a recombinant protein spanning amino acids 10–90 of human RPL35A, a region conserved across mouse and rat; consider this when verifying cross-reactivity in these species.
• Application suitability: Validated for western blotting (WB) and ELISA; for WB, expect a band near 13 kDa; due to polyclonal nature, verify specificity in knockout or knockdown samples if available.
• Cross-reactivity: Detects mouse and rat RPL35A; for other species, check sequence homology before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers and research labs by offering validated antibodies, recombinant proteins, and ancillary reagents. This anti-RPL35A polyclonal antibody supports ribosome biology and hematological disorder research. Beyond this product, CamelBio can source matched antibody pairs, monoclonal alternatives, and rare-target raw materials to accelerate your assay development from concept to clinic.