RPL28 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P46779

Core Product Specifications and Parameters

Biological Background: RPL28 Function and Localization

• RPL28 encodes the 60S ribosomal protein L28, a core component of the large ribosomal subunit essential for mRNA translation and protein synthesis. Related references: PMID:12962325, PMID:23636399, PMID:32669547
• The RPL28 protein and its associated ribosome predominantly localize to the cytoplasm, where ribosome biogenesis and protein synthesis occur.
• RPL28 belongs to the eukaryotic ribosomal protein eL28 family, characterized by conserved structural domains required for ribosome assembly.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, potentially regulating ribosome function or turnover.
• Alternative splicing of the RPL28 gene produces multiple transcript variants, which may contribute to isoform diversity.
• Keywords: 3D-structure; Acetylation; Alternative splicing; Cytoplasm; Direct protein sequencing; Isopeptide bond; Phosphoprotein; Proteomics identification; Reference proteome; Ribonucleoprotein; Ribosomal protein; Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (aa 1-100). This antibody is expected to recognize the full-length RPL28 protein (approx. 16 kDa) and may detect isoforms if the epitope is conserved.
• For Western blot (WB), a band near 16 kDa is anticipated in lysates from human, mouse, or rat cells. Consider using appropriate positive controls (e.g., HeLa, HEK293, or liver tissue) and validate the observed band size against a molecular weight marker.
• For immunofluorescence/immunocytochemistry (IF/ICC), expect cytoplasmic staining consistent with ribosomal localization. Include a nuclear counterstain to confirm subcellular distribution.
• ELISA applications should be optimized with the appropriate peptide or protein coating concentration; check cross-reactivity with other ribosomal proteins if needed.
• As with any polyclonal antibody, lot-to-lot variability may exist; we recommend validating each new lot in the target assay system and considering affinity purification for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with one-stop access to IVD raw materials and technical support—from concept to clinic. For ribosomal targets like RPL28, we supply validated polyclonal antibodies, antibody pairs, and ancillary reagents in bulk to streamline your assay development. Our sourcing network also covers rare and emerging biomarkers, ensuring you secure the right raw materials for your diagnostic pipeline.