RPB3/POLR2C Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P19387

Core Product Specifications and Parameters

Biological Background: RPB3/POLR2C Function and Localization

• DNA-directed RNA polymerase II subunit RPB3, also known as POLR2C, RPB31, or hRPB33, is a core component of RNA polymerase II (Pol II).
• Pol II is responsible for synthesizing mRNA precursors and many functional non-coding RNAs from DNA templates using ribonucleoside triphosphates.
• RPB3 is part of the Pol II core complex, essential for transcription initiation, elongation, and termination.
• The protein localizes to the nucleus, consistent with its role in transcription.
• Post-translational modifications include phosphorylation (keyword: Phosphoprotein), which may regulate activity or complex assembly.
• The protein has a molecular weight of approximately 31.4 kDa (31441 Da) and is evolutionarily conserved among eukaryotes.
• Structural studies (keyword: 3D-structure) have revealed its arrangement within the Pol II complex.
• It is a reference proteome member and has been identified via direct protein sequencing and proteomics.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human RPB3/POLR2C, which may contribute to specific recognition. Consider validating antibody specificity in knockout or knockdown experiments when possible.
• For Western blot, the expected band size is approximately 31 kDa; use appropriate loading controls and verify band specificity.
• Immunohistochemistry (IHC-P) may require antigen retrieval optimization; test on known positive control tissues.
• Cross-reactivity with mouse and rat suggests potential use in these species, but validate in relevant sample systems.
• ELISA applications may require titration of both capture and detection antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing. For transcription and RNA polymerase II research, we provide reliable access to critical reagents like the RPB3/POLR2C antibody, supporting your diagnostic development from concept to clinic with efficient procurement and supply-chain expertise.