RPAP3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H6T3

Core Product Specifications and Parameters

Biological Background: RPAP3 Function and Localization

• RPAP3 (RNA polymerase II-associated protein 3) is a 76 kDa protein also known as Tah1 or hSpagh, encoded by the RPAP3 gene on human chromosome 12.
• It functions as an interface between the RNA polymerase II enzyme and chaperone/scaffolding proteins, bridging RNA polymerase II to regulators of protein complex formation.
• RPAP3 belongs to the TPR (tetratricopeptide repeat) repeat-containing protein family, which mediates protein-protein interactions.
• The protein undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitin-like conjugation (Ubl), as indicated by proteomics data.
• Alternative splicing generates multiple isoforms, and the protein has a solved 3D structure.
• Keywords include reference proteome and isopeptide bond formation.

Experimental Guidance and Technical Tips

• For western blot, the antibody is recommended for detection of RPAP3 in human, mouse, and rat lysates; expect a band near 76 kDa.
• For immunofluorescence/ICC, validate fixation and permeabilization conditions in your cell system.
• For ELISA, use as a capture or detection antibody according to your assay design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like RPAP3, a key component in RNA polymerase II complex regulation, we offer optimized polyclonal antibodies and can assist with rare target sourcing to accelerate your assay development from concept to clinic.