ROMO1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P60602

Core Product Specifications and Parameters

Biological Background: ROMO1 Function and Localization

• ROMO1 (Reactive oxygen species modulator 1), also known as MTGM, Glyrichin, or Protein MGR2 homolog, is a mitochondrial inner membrane protein encoded by the ROMO1 gene (C20orf52).
• It induces production of reactive oxygen species (ROS) necessary for cell proliferation, and may contribute to oxidative DNA damage and replicative senescence.
• ROMO1 exhibits antibacterial activity against various bacteria, including S. aureus, P. aeruginosa, and M. tuberculosis, by inducing bacterial membrane breakage.
• The protein localizes to the mitochondrial inner membrane.
• ROMO1 is up-regulated in multiple cancer cell lines compared to normal lung fibroblast cells and is highly expressed in brain tumors, suggesting a role in cancer biology.
• Keywords associated with ROMO1 include: Alternative splicing, Antibiotic, Antimicrobial, Membrane, Mitochondrion, Mitochondrion inner membrane, Proteomics identification, Reference proteome, Transmembrane, Transmembrane helix.
• The ROMO1 protein has a predicted molecular weight of approximately 8.18 kDa (UniProt: 8183 Da) and runs at ~8 kDa in SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (aa 1–79) of human ROMO1; ensure target epitope conservation in your sample species (human, mouse, rat).
• For Western blot, the predicted molecular weight is ~8 kDa; use a high-percentage gel (e.g., 15%) and short transfer times to prevent loss of small proteins.
• This antibody is suitable for immunofluorescence on paraffin-embedded (IF-P) sections; optimize antigen retrieval and permeabilization for mitochondrial targets.
• ROMO1 is overexpressed in many cancer cell lines and brain tumors; consider using these as positive controls.
• For ELISA, validate performance with recombinant ROMO1 or cell lysates under your experimental conditions.
• Avoid bacterial contamination in samples due to ROMO1's antimicrobial activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD manufacturers and researchers, offering high-quality raw materials like this ROMO1 polyclonal antibody for mitochondrial and cancer research. Beyond catalog products, we provide customized antibody development, bulk reagent supply, and sourcing of rare targets to streamline your diagnostic and assay development. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies, or ancillary reagents, CamelBio bridges the gap from concept to clinic.