ROCK2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O75116

Core Product Specifications and Parameters

Biological Background: ROCK2 Function and Localization

• ROCK2 (Rho-associated protein kinase 2, also known as ROCK-II or p164 ROCK-2) is a serine/threonine-protein kinase encoded by the ROCK2 gene.
• It is a key regulator of the actin cytoskeleton and cell polarity, involved in smooth muscle contraction, stress fiber and focal adhesion formation, neurite retraction, cell adhesion, and motility.
• ROCK2 phosphorylates a variety of substrates including ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A, VIM, SORL1, and IRF4, mediating diverse cellular processes.
• The protein acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation and positively regulates MAPK1/3 and p90RSK during myogenic differentiation.
• It plays roles in centrosome duplication, keratinocyte terminal differentiation inhibition, and may regulate eyelid and ventral body wall closure via actomyosin organization.
• In the hippocampus, ROCK2 is critical for the regulation of spine and synaptic properties, and it also contributes to the circadian rhythm of aortic myofilament Ca²⁺ sensitivity and vascular contractility.
• Subcellularly, ROCK2 localizes to the cytoplasm, cell membrane, nucleus, cytoskeleton, microtubule organizing center, and centrosome.
• The protein contains coiled-coil and zinc-finger domains, and its molecular weight is approximately 160.9 kDa; it is expressed in the brain and classified under phosphoprotein.

Experimental Guidance and Technical Tips

• For Western blotting, consider using a low-percentage (e.g., 6-8%) SDS-PAGE gel to effectively resolve the large ~161 kDa ROCK2 protein, and ensure adequate transfer time to high-molecular-weight membranes.
• In immunohistochemistry (paraffin), antigen retrieval conditions may need empirical optimization; test citric acid or EDTA-based methods depending on tissue type.
• Validate antibody performance in positive control lysates from human, mouse, or rat cell lines known to express ROCK2 (e.g., HeLa, NIH/3T3, or brain tissues) before experimental use.
• ELISA protocols may benefit from peptide blocking experiments to confirm specificity; titrate the antibody for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research professionals by providing reliable IVD raw materials, including validated monoclonal antibodies like this ROCK2 clone. Whether you need bulk quantities of this antibody, custom formulations, or complementary reagents for actin cytoskeleton and kinase signaling research, CamelBio streamlines your procurement process. Our team can also source rare-target materials and ancillary reagents to accelerate your assay development from concept to clinic.